Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2032611..2033113 Replicon chromosome
Accession NZ_CP012693
Organism Escherichia coli strain FORC_028

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FORC28_RS10180 Protein ID WP_000767829.1
Coordinates 2032859..2033113 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FORC28_RS10175 Protein ID WP_001259255.1
Coordinates 2032611..2032862 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC28_RS10155 2027792..2028859 - 1068 WP_000080075.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FORC28_RS10160 2028859..2029929 - 1071 WP_000108914.1 histidinol-phosphate transaminase -
FORC28_RS10165 2029926..2031230 - 1305 WP_000009589.1 histidinol dehydrogenase -
FORC28_RS10170 2031236..2032135 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FORC28_RS32990 2032281..2032328 - 48 WP_001375657.1 his operon leader peptide -
FORC28_RS10175 2032611..2032862 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FORC28_RS10180 2032859..2033113 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FORC28_RS10185 2033196..2034020 + 825 WP_000754737.1 SDR family oxidoreductase -
FORC28_RS10190 2034066..2034995 + 930 WP_000803351.1 LysR family transcriptional regulator -
FORC28_RS32995 2035210..2035272 + 63 WP_010723108.1 membrane protein YoeI -
FORC28_RS10195 2035262..2036620 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FORC28_RS10200 2036799..2037857 + 1059 WP_000492324.1 transport protein YeeE -
FORC28_RS10205 2037871..2038098 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T57006 WP_000767829.1 NZ_CP012693:2032859-2033113 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T57006 NZ_CP012693:2032859-2033113 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT57006 WP_001259255.1 NZ_CP012693:2032611-2032862 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT57006 NZ_CP012693:2032611-2032862 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References