Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1160403..1161130 Replicon chromosome
Accession NZ_CP012693
Organism Escherichia coli strain FORC_028

Toxin (Protein)


Gene name higB Uniprot ID I2UEW4
Locus tag FORC28_RS05755 Protein ID WP_000547559.1
Coordinates 1160403..1160714 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag FORC28_RS05760 Protein ID WP_000126294.1
Coordinates 1160711..1161130 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC28_RS05725 1155545..1157254 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
FORC28_RS05730 1157264..1157806 + 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
FORC28_RS05735 1157806..1158573 + 768 WP_000067401.1 formate hydrogenlyase subunit HycG -
FORC28_RS05740 1158570..1158980 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
FORC28_RS05745 1158973..1159443 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
FORC28_RS05750 1159486..1160229 + 744 WP_001341829.1 hypothetical protein -
FORC28_RS05755 1160403..1160714 + 312 WP_000547559.1 type II toxin-antitoxin system HigB family toxin Toxin
FORC28_RS05760 1160711..1161130 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
FORC28_RS05765 1161244..1162668 - 1425 WP_000110313.1 6-phospho-beta-glucosidase AscB -
FORC28_RS05770 1162677..1164134 - 1458 WP_001107856.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
FORC28_RS05775 1164394..1165404 + 1011 WP_001377591.1 DNA-binding transcriptional regulator AscG -
FORC28_RS05780 1165553..1166080 + 528 WP_001078781.1 electron transport protein HydN -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12450.11 Da        Isoelectric Point: 9.7249

>T57004 WP_000547559.1 NZ_CP012693:1160403-1160714 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T57004 NZ_CP012693:1160403-1160714 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT57004 WP_000126294.1 NZ_CP012693:1160711-1161130 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT57004 NZ_CP012693:1160711-1161130 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0F3V163


Antitoxin

Source ID Structure

References