Detailed information of TA system TA056979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 103950..104587
• Replicon: plasmid unnamed
• Accession: NZ_CP012688
• Organism: Ralstonia solanacearum strain UY031

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1C0UHE0
• Locus tag: RSUY_RS16135
• Protein ID: WP_003262800.1
• Coordinates: 103950..104279 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RSUY_RS16140
• Protein ID: WP_003262799.1
• Coordinates: 104276..104587 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSUY_RS16120	99659..100822	-	1164	WP_003262804.1	2-methylcitrate synthase	-
RSUY_RS16125	100860..101756	-	897	WP_020957574.1	methylisocitrate lyase	-
RSUY_RS16130	101950..103899	+	1950	WP_039558250.1	propionate catabolism operon regulatory protein PrpR	-
RSUY_RS16135	103950..104279	+	330	WP_003262800.1	hypothetical protein	Toxin
RSUY_RS16140	104276..104587	+	312	WP_003262799.1	transcriptional regulator	Antitoxin
RSUY_RS16145	104819..105127	-	309	WP_003262797.1	type II toxin-antitoxin system YafQ family toxin	-
RSUY_RS16150	105072..105383	-	312	WP_003262795.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
RSUY_RS16155	105967..106800	-	834	WP_039558252.1	DUF2242 domain-containing protein	-
RSUY_RS16160	107082..107453	+	372	WP_020957571.1	hypothetical protein	-
RSUY_RS16165	107646..108386	-	741	WP_003262792.1	metallophosphatase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgG / fliM / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1999545	1999545
	-	flank	IS/Tn	-	-	108733..109719	986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12457.90 Da
• Isoelectric Point: 4.9855

>T56979 WP_003262800.1 NZ_CP012688:103950-104279 [Ralstonia solanacearum]
MDATPIELPGEHDLSDDEYSALQQELLAQPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGSSQFWMFVVYD
KDEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 330 bp

>T56979 NZ_CP012688:103950-104279 [Ralstonia solanacearum]
ATGGACGCTACCCCCATTGAGTTGCCGGGGGAGCACGATCTCTCGGACGACGAGTACAGCGCCCTTCAACAGGAACTGCT
CGCCCAGCCGGACGCTGGCGACGTCATCAAGGGGACAGGTGGTCTACGCAAGCTGCGGTTTTCGGACAAGCGGCGCGGCA
AAGGTAAGCGCGGCGGTCTGCGCGTCATCTATTACCACTGGGATGGAAGCAGCCAATTCTGGATGTTTGTCGTCTACGAC
AAAGACGAGGCGGACGATCTTTCCAGTGACGAGCGGAAAGTGCTGGCACGGCTGTTGGAGCAGGAAGTCAAGGCAAGGAG
TGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11620.59 Da
• Isoelectric Point: 10.3969

>AT56979 WP_003262799.1 NZ_CP012688:104276-104587 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVEVRVLQPIDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT56979 NZ_CP012688:104276-104587 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGACGCGCTGGCCGCAGAGCGCGAAGGAAAGATGACCCTCCG
CAAGGTCGAGGTCCGCGTGCTCCAGCCGATTGATGTCACCGCCGAGGAAATCAAGGCCGTTCGCGCAGCGGCCCATGCCT
CACAGGCTGTCATGGCACGTCGGCTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGCACCGCCAAACCGAAC
ACCCAGGCGGCCGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A1C0UHE0