Detailed information of TA system TA056972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3944816..3945546
• Replicon: chromosome
• Accession: NZ_CP012685
• Organism: Serratia marcescens strain SmUNAM836

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2S4X738
• Locus tag: ABH11_RS18595
• Protein ID: WP_033636137.1
• Coordinates: 3944816..3945127 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ABH11_RS18600
• Protein ID: WP_060431405.1
• Coordinates: 3945124..3945546 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ABH11_RS18565	3940127..3940852	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
ABH11_RS18570	3940905..3942338	+	1434	WP_033648997.1	RNA polymerase factor sigma-54	-
ABH11_RS18575	3942362..3942649	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
ABH11_RS18580	3942805..3943266	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
ABH11_RS18585	3943396..3944250	+	855	WP_015379177.1	RNase adapter RapZ	-
ABH11_RS18590	3944247..3944519	+	273	WP_033648996.1	PTS phosphocarrier protein NPr	-
ABH11_RS18595	3944816..3945127	+	312	WP_033636137.1	type II toxin-antitoxin system HigB family toxin	Toxin
ABH11_RS18600	3945124..3945546	+	423	WP_060431405.1	helix-turn-helix domain-containing protein	Antitoxin
ABH11_RS18605	3945551..3947467	-	1917	WP_060706844.1	BglG family transcription antiterminator	-
ABH11_RS18610	3947519..3948733	-	1215	WP_060706845.1	lactonase family protein	-
ABH11_RS18615	3948948..3949688	-	741	WP_060706846.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12259.11 Da
• Isoelectric Point: 10.0957

>T56972 WP_033636137.1 NZ_CP012685:3944816-3945127 [Serratia marcescens]
MHIISREPFNNATKQYPNEAAALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T56972 NZ_CP012685:3944816-3945127 [Serratia marcescens]
ATGCATATCATTTCCAGGGAGCCGTTCAATAACGCAACCAAGCAATACCCTAATGAAGCCGCTGCTCTCGAGGCCATTTA
TAAAGCGTTGCGACGCGGCGAGTTCCGCACGCCGGACGAGTTAAAAGCGCTTTTTCCTTCGCTCGATCGTATGAAATACA
AAGAGAAATGGTGGGTCATTGACGTCGGCGGCAACCACCTGCGGATTTTGTTCTTCGCCAGCTTCGAGACACAGAAAGTC
TTCATAAAGCATATCGTTAGCCACGCCGAGTACGACAAGCTGATGCAGCACTATCGAAGGAGTTCATCATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15704.98 Da
• Isoelectric Point: 4.9590

>AT56972 WP_060431405.1 NZ_CP012685:3945124-3945546 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSSSQQDYREALELVEYLLENDEHHPLIDMLAKRIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVSQILSGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT56972 NZ_CP012685:3945124-3945546 [Serratia marcescens]
ATGATTACAGATGCCATTAAGGCCGCCGATGCGCTGCTGCGCGCCGTGCCCTTATTGCAGGGCAGCAGCTCTCAGCAAGA
CTACCGGGAAGCGCTCGAATTGGTGGAATATTTGCTGGAAAACGATGAACACCATCCGCTGATTGACATGCTGGCAAAAA
GAATCGCCGAATATGAAGATAACAGCGCCGAATTTACCGCATTCAACCAGCGTATCGCCCAACTCCCGCAAGGAGTGGCC
GCGCTTCGCGTATTGATGGAACAACACCAACTTAGCCAAAGTGATTTTGAACATGAGATCGGTAAAAAGTCGTTGGTCAG
CCAAATCCTGAGTGGCAAAAGATCGCTGACCATTCCGCACATTATGGCGCTGGCCAAACGCTTCAATCTGCCCCCGGCCC
TCTTCCTGCCGGAAGCGGATTAG

Structures

Toxin

• AlphaFold DB: A0A2S4X738