Detailed information of TA system TA056952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5910792..5911447
• Replicon: chromosome
• Accession: NZ_CP012680
• Organism: Pseudomonas brassicacearum strain LBUM300

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G8QAX9
• Locus tag: AK973_RS25750
• Protein ID: WP_013694185.1
• Coordinates: 5911103..5911447 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AK973_RS25745
• Protein ID: WP_003205391.1
• Coordinates: 5910792..5911106 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AK973_RS25725	5906838..5907755	+	918	WP_013694183.1	LysR family transcriptional regulator	-
AK973_RS25730	5907905..5908918	+	1014	WP_003205388.1	diguanylate cyclase	-
AK973_RS25735	5909010..5909753	+	744	WP_013694184.1	DUF72 domain-containing protein	-
AK973_RS25740	5909875..5910579	+	705	WP_003205390.1	hypothetical protein	-
AK973_RS25745	5910792..5911106	-	315	WP_003205391.1	helix-turn-helix domain-containing protein	Antitoxin
AK973_RS25750	5911103..5911447	-	345	WP_013694185.1	hypothetical protein	Toxin
AK973_RS25755	5911587..5912474	-	888	WP_003205393.1	heme o synthase	-
AK973_RS25760	5912486..5912821	-	336	WP_003205394.1	cytochrome o ubiquinol oxidase subunit IV	-
AK973_RS25765	5912822..5913448	-	627	WP_003205395.1	cytochrome o ubiquinol oxidase subunit III	-
AK973_RS25770	5913452..5915482	-	2031	WP_003205396.1	cytochrome o ubiquinol oxidase subunit I	-
AK973_RS25775	5915486..5916433	-	948	WP_003205397.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13753.72 Da
• Isoelectric Point: 8.3860

>T56952 WP_013694185.1 NZ_CP012680:c5911447-5911103 [Pseudomonas brassicacearum]
MRTLFFETTTFTDTVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYDKDELENLTSEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T56952 NZ_CP012680:c5911447-5911103 [Pseudomonas brassicacearum]
ATGCGTACCCTATTTTTTGAAACGACTACATTCACCGACACGGTTGGCCATTACCTGACTGATGATGAGTATCGCCTGCT
TCAGTCCTATATGCTGCAGCATCCAGAGGTTGGTGACGTCATGCCACGTACCGGCGGCTTTCGTAAGCTGCGCTGGTTCG
ATGAACGTCGTGGTAAAGGGAAGCGAGGCGGGTTGCGCGTTATTTACTATTGGCTCATGAACGATCGTCAGTTCTGGATG
TTCGCGATTTATGACAAGGACGAGTTGGAAAACCTGACCTCCGAGCAAGAGAAGACGCTCAAACGCGCCATAGAAGCTGA
GTTGAAAGTACGAGGTACCTTATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.76 Da
• Isoelectric Point: 9.8409

>AT56952 WP_003205391.1 NZ_CP012680:c5911106-5910792 [Pseudomonas brassicacearum]
MKKRDLFAEMMQGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIKLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT56952 NZ_CP012680:c5911106-5910792 [Pseudomonas brassicacearum]
ATGAAAAAGCGCGATCTGTTTGCCGAGATGATGCAAGGCGTCGAGGAGATGGCCGCCCACCGGGAGGGCAAGATTACCCT
TCGCCAGATATCGATCGAAGACAAACCGGTTCCCGAGGTGTCTGCCCAAGAAATCGTGGCGTTGCGCGACAAGCTCCACA
TGTCTCAAGCCGTTTTCGCCAAGAGCATCAGGACCAGTCCAGGTACGCTCAGGAACTGGGAGCAGGAGAAGTCCAAGCCT
AACGCCCAGGCGGCTTTGTTGATCAAGCTGGTCGAGAAGTTTCCAGATATGGTCGAACGGCTTGGCGCTGTTTGA

Structures

Toxin

• AlphaFold DB: G8QAX9