Detailed information of TA system TA056951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5468283..5468878
• Replicon: chromosome
• Accession: NZ_CP012679
• Organism: Pseudomonas aeruginosa strain PA1RG

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: AOD73_RS31065
• Protein ID: WP_003113526.1
• Coordinates: 5468600..5468878 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AOD73_RS25420
• Protein ID: WP_003099268.1
• Coordinates: 5468283..5468588 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOD73_RS25385	5463423..5464271	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
AOD73_RS25395	5464438..5465379	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
AOD73_RS25400	5465496..5466110	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
AOD73_RS25405	5466152..5466736	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
AOD73_RS25410	5466777..5467877	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
AOD73_RS25420	5468283..5468588	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
AOD73_RS31065	5468600..5468878	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AOD73_RS25425	5469207..5471435	+	2229	WP_003099265.1	TonB-dependent receptor	-
AOD73_RS25430	5471505..5472152	-	648	WP_003095021.1	carbonate dehydratase	-
AOD73_RS25435	5472214..5473452	-	1239	WP_009316205.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T56951 WP_003113526.1 NZ_CP012679:c5468878-5468600 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T56951 NZ_CP012679:c5468878-5468600 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT56951 WP_003099268.1 NZ_CP012679:c5468588-5468283 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT56951 NZ_CP012679:c5468588-5468283 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3