Detailed information of TA system TA056926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 749528..750126
• Replicon: chromosome
• Accession: NZ_CP012667
• Organism: Vibrio cholerae strain ICDC-VC661

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q8KQX9
• Locus tag: AN947_RS09000
• Protein ID: WP_000118545.1
• Coordinates: 749803..750126 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AN947_RS08995
• Protein ID: WP_000058216.1
• Coordinates: 749528..749806 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN947_RS08980	746490..748310	+	1821	WP_000661887.1	conjugative transfer system coupling protein TraD	-
AN947_RS08985	748320..748880	+	561	WP_000546510.1	hypothetical protein	-
AN947_RS08990	748867..749502	+	636	WP_000033753.1	DUF4400 domain-containing protein	-
AN947_RS08995	749528..749806	-	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
AN947_RS09000	749803..750126	-	324	WP_000118545.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AN947_RS09005	750307..750588	+	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
AN947_RS09010	750585..751211	+	627	WP_000667169.1	hypothetical protein	-
AN947_RS09015	751195..752091	+	897	WP_032467354.1	type-F conjugative transfer system secretin TraK	-
AN947_RS09020	752094..753383	+	1290	WP_001883765.1	TraB/VirB10 family protein	-
AN947_RS09025	753458..754030	+	573	WP_139057167.1	type IV conjugative transfer system lipoprotein TraV	-
AN947_RS09030	754027..754413	+	387	WP_000180225.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12267.26 Da
• Isoelectric Point: 9.4705

>T56926 WP_000118545.1 NZ_CP012667:c750126-749803 [Vibrio cholerae]
MSWKIDFYDGVEDQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGNHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T56926 NZ_CP012667:c750126-749803 [Vibrio cholerae]
ATGAGCTGGAAGATCGACTTTTACGATGGCGTTGAGGATCAGATCCTCGATATGCCACCCAAAATACAAGCTCGCATGAT
TAAGCTTTTAGAGCTGATGGAAAAGCATGGTGCAAACTTGGGGCCTCCTCATACTGAGTCTATGGGCGATGGGTTATTTG
AAATCCGCGCCAAAGCTCAAGAAGGTATTGGTAGAGGCTTATTTTGCTACTTAAAAGGGAATCACATTTATGTGTTACAC
GCTTTTGTAAAAAAATCTCAAAAGACGCCCAAAAATGAGCTCAATCTTGCCAGAGATAGACAAAAAGAGGTTCAAAAATC
ATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT56926 WP_000058216.1 NZ_CP012667:c749806-749528 [Vibrio cholerae]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT56926 NZ_CP012667:c749806-749528 [Vibrio cholerae]
ATGAGTCTGCAAAAACTGAAACAACGTGCTTTAGCCAATGCTGAAGTTAAGAATGAGTACGATAAGCTTGAGAGTGAGTT
CAGCTTTATTGATCAATTGCTTACCATGAGAACAAAGGCAGGACTTACTCAAGAACAAGTTGCTGAAAGAATGCATACCC
AAAAAAGTAATGTCAGTCGTTTAGAAAAAGGTAATGCCAATCCAAGTTGGTCAACTCTTCTAAAATATGCTCACGCATGT
GGCTTTGAACTAACACTCAAGCCACAAAAAATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A0X1L383