Detailed information of TA system TA056899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4833324..4833926
• Replicon: chromosome
• Accession: NZ_CP012635
• Organism: Escherichia coli strain SF-088

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AN203_RS24360
• Protein ID: WP_000897302.1
• Coordinates: 4833615..4833926 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AN203_RS24355
• Protein ID: WP_000356397.1
• Coordinates: 4833324..4833614 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN203_RS24325	4829397..4830299	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AN203_RS24330	4830296..4830931	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AN203_RS24335	4830928..4831857	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AN203_RS24340	4832073..4832291	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
AN203_RS24345	4832687..4832965	-	279	WP_001296612.1	hypothetical protein	-
AN203_RS24355	4833324..4833614	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AN203_RS24360	4833615..4833926	-	312	WP_000897302.1	hypothetical protein	Toxin
AN203_RS24365	4834155..4835063	+	909	WP_001331553.1	alpha/beta hydrolase	-
AN203_RS24370	4835127..4836068	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AN203_RS24375	4836113..4836550	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AN203_RS24380	4836547..4837419	-	873	WP_000920754.1	virulence factor BrkB family protein	-
AN203_RS24385	4837413..4838012	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T56899 WP_000897302.1 NZ_CP012635:c4833926-4833615 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T56899 NZ_CP012635:c4833926-4833615 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT56899 WP_000356397.1 NZ_CP012635:c4833614-4833324 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT56899 NZ_CP012635:c4833614-4833324 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416