Detailed information of TA system TA056891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3751710..3752404
• Replicon: chromosome
• Accession: NZ_CP012635
• Organism: Escherichia coli strain SF-088

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AN203_RS18850
• Protein ID: WP_001263500.1
• Coordinates: 3751710..3752108 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AN203_RS18855
• Protein ID: WP_000554758.1
• Coordinates: 3752111..3752404 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN203_RS18825	3747075..3747533	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
AN203_RS18830	3747794..3749251	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
AN203_RS18835	3749308..3749922	-	615	WP_000602123.1	peptide chain release factor H	-
AN203_RS18840	3749919..3751058	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
AN203_RS18845	3751248..3751700	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
AN203_RS18850	3751710..3752108	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AN203_RS18855	3752111..3752404	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AN203_RS18860	3752456..3753511	-	1056	WP_001226168.1	DNA polymerase IV	-
AN203_RS18865	3753582..3754367	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
AN203_RS18870	3754339..3756051	+	1713	Protein_3590	flagellar biosynthesis protein FlhA	-
AN203_RS30080	3756130..3756288	+	159	WP_014639450.1	hypothetical protein	-
AN203_RS18875	3756371..3756868	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3751710..3772064	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T56891 WP_001263500.1 NZ_CP012635:c3752108-3751710 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T56891 NZ_CP012635:c3752108-3751710 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT56891 WP_000554758.1 NZ_CP012635:c3752404-3752111 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT56891 NZ_CP012635:c3752404-3752111 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3