Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3508883..3509562 Replicon chromosome
Accession NZ_CP012635
Organism Escherichia coli strain SF-088

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag AN203_RS17700 Protein ID WP_000057524.1
Coordinates 3509260..3509562 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag AN203_RS17695 Protein ID WP_000806442.1
Coordinates 3508883..3509224 (-) Length 114 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN203_RS17685 3505127..3506059 - 933 WP_000883052.1 glutaminase A -
AN203_RS17690 3506321..3508825 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
AN203_RS17695 3508883..3509224 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
AN203_RS17700 3509260..3509562 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AN203_RS17705 3509695..3510489 + 795 WP_000365177.1 TraB/GumN family protein -
AN203_RS17710 3510693..3511172 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
AN203_RS17715 3511196..3511996 + 801 WP_000439796.1 hypothetical protein -
AN203_RS17720 3511993..3512496 + 504 WP_000667000.1 hypothetical protein -
AN203_RS17725 3512534..3514186 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3501572..3512496 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(9-98)

Antitoxin

(1-100)


Sequences


Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T56889 WP_000057524.1 NZ_CP012635:c3509562-3509260 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T56889 NZ_CP012635:c3509562-3509260 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT56889 WP_000806442.1 NZ_CP012635:c3509224-3508883 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT56889 NZ_CP012635:c3509224-3508883 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0VBP6


Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References