Detailed information of TA system TA056864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3714653..3715347
• Replicon: chromosome
• Accession: NZ_CP012633
• Organism: Escherichia coli strain SF-166

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: AN204_RS18275
• Protein ID: WP_001263500.1
• Coordinates: 3714653..3715051 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: AN204_RS18280
• Protein ID: WP_000554758.1
• Coordinates: 3715054..3715347 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN204_RS18250	3710018..3710476	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
AN204_RS18255	3710737..3712194	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
AN204_RS18260	3712251..3712865	-	615	WP_000602123.1	peptide chain release factor H	-
AN204_RS18265	3712862..3714001	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
AN204_RS18270	3714191..3714643	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
AN204_RS18275	3714653..3715051	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AN204_RS18280	3715054..3715347	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AN204_RS18285	3715399..3716454	-	1056	WP_001226168.1	DNA polymerase IV	-
AN204_RS18290	3716525..3717310	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
AN204_RS18295	3717282..3718994	+	1713	Protein_3491	flagellar biosynthesis protein FlhA	-
AN204_RS28080	3719073..3719231	+	159	WP_014639450.1	hypothetical protein	-
AN204_RS18300	3719314..3719811	-	498	WP_001557224.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3714653..3735007	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T56864 WP_001263500.1 NZ_CP012633:c3715051-3714653 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T56864 NZ_CP012633:c3715051-3714653 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT56864 WP_000554758.1 NZ_CP012633:c3715347-3715054 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT56864 NZ_CP012633:c3715347-3715054 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3