Detailed information of TA system TA056842

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20401..20827
• Replicon: plasmid pSF-173-1
• Accession: NZ_CP012632
• Organism: Escherichia coli strain SF-173

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AN205_RS25215
• Protein ID: WP_001312861.1
• Coordinates: 20401..20559 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	20603..20827 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN205_RS25180	15738..15977	-	240	WP_042018283.1	conjugal transfer relaxosome protein TraY	-
AN205_RS25185	16085..16732	-	648	WP_053811576.1	transcriptional regulator TraJ family protein	-
AN205_RS25190	16924..17307	-	384	WP_001151529.1	relaxosome protein TraM	-
AN205_RS25195	17583..18230	+	648	WP_000601040.1	transglycosylase SLT domain-containing protein	-
AN205_RS25200	18526..19347	-	822	WP_001234469.1	DUF945 domain-containing protein	-
AN205_RS25205	19467..19754	-	288	WP_000107535.1	hypothetical protein	-
AN205_RS28770	19756..19953	+	198	Protein_29	hypothetical protein	-
AN205_RS25215	20401..20559	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	20603..20827	-	225	NuclAT_0	-	Antitoxin
-	20603..20827	-	225	NuclAT_0	-	Antitoxin
-	20603..20827	-	225	NuclAT_0	-	Antitoxin
-	20603..20827	-	225	NuclAT_0	-	Antitoxin
AN205_RS28870	20639..20827	+	189	WP_001336239.1	hypothetical protein	-
AN205_RS25225	20839..21558	-	720	WP_001276234.1	plasmid SOS inhibition protein A	-
AN205_RS25230	21555..21989	-	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
AN205_RS25235	22044..24002	-	1959	WP_053811577.1	ParB/RepB/Spo0J family partition protein	-
AN205_RS25240	24067..24300	-	234	WP_000006030.1	DUF905 family protein	-
AN205_RS25245	24362..24901	-	540	WP_001530505.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C / mph(A) / sul1 / qacE / aadA2 / dfrA12	-	1..93074	93074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T56842 WP_001312861.1 NZ_CP012632:c20559-20401 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T56842 NZ_CP012632:c20559-20401 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT56842 NZ_CP012632:c20827-20603 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGTCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin