Detailed information of TA system TA056814

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56629..56893
• Replicon: plasmid pSF-468-2
• Accession: NZ_CP012627
• Organism: Escherichia coli strain SF-468

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: AN206_RS26955
• Protein ID: WP_001331364.1
• Coordinates: 56629..56781 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	56836..56893 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN206_RS26925	51906..54074	+	2169	WP_000698357.1	DotA/TraY family protein	-
AN206_RS26930	54148..54798	+	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
AN206_RS26935	54870..55079	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
AN206_RS31080	55471..55647	+	177	WP_001054898.1	hypothetical protein	-
AN206_RS26950	56306..56557	+	252	WP_001291964.1	hypothetical protein	-
AN206_RS26955	56629..56781	-	153	WP_001331364.1	Hok/Gef family protein	Toxin
-	56836..56893	+	58	NuclAT_0	-	Antitoxin
-	56836..56893	+	58	NuclAT_0	-	Antitoxin
-	56836..56893	+	58	NuclAT_0	-	Antitoxin
-	56836..56893	+	58	NuclAT_0	-	Antitoxin
-	57134..57189	+	56	NuclAT_1	-	-
-	57134..57189	+	56	NuclAT_1	-	-
-	57134..57189	+	56	NuclAT_1	-	-
-	57134..57189	+	56	NuclAT_1	-	-
AN206_RS26960	57350..58336	+	987	WP_001257838.1	hypothetical protein	-
AN206_RS26965	58554..59762	+	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
AN206_RS26970	59781..60851	+	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..92766	92766

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T56814 WP_001331364.1 NZ_CP012627:c56781-56629 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T56814 NZ_CP012627:c56781-56629 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT56814 NZ_CP012627:56836-56893 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin