Detailed information of TA system TA056813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4929217..4929819
• Replicon: chromosome
• Accession: NZ_CP012625
• Organism: Escherichia coli strain SF-468

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AN206_RS24970
• Protein ID: WP_000897302.1
• Coordinates: 4929508..4929819 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AN206_RS24965
• Protein ID: WP_000356397.1
• Coordinates: 4929217..4929507 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN206_RS24935	4925290..4926192	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
AN206_RS24940	4926189..4926824	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AN206_RS24945	4926821..4927750	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AN206_RS24950	4927966..4928184	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
AN206_RS24955	4928580..4928858	-	279	WP_001296612.1	hypothetical protein	-
AN206_RS24965	4929217..4929507	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AN206_RS24970	4929508..4929819	-	312	WP_000897302.1	hypothetical protein	Toxin
AN206_RS24975	4930048..4930956	+	909	WP_001331553.1	alpha/beta hydrolase	-
AN206_RS24980	4931020..4931961	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AN206_RS24985	4932006..4932443	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AN206_RS24990	4932440..4933312	-	873	WP_000920754.1	virulence factor BrkB family protein	-
AN206_RS24995	4933306..4933905	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T56813 WP_000897302.1 NZ_CP012625:c4929819-4929508 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T56813 NZ_CP012625:c4929819-4929508 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT56813 WP_000356397.1 NZ_CP012625:c4929507-4929217 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT56813 NZ_CP012625:c4929507-4929217 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416