Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3968541..3969235 Replicon chromosome
Accession NZ_CP012625
Organism Escherichia coli strain SF-468

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag AN206_RS20280 Protein ID WP_001263500.1
Coordinates 3968541..3968939 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag AN206_RS20285 Protein ID WP_000554758.1
Coordinates 3968942..3969235 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN206_RS20255 3963906..3964364 - 459 WP_001291988.1 xanthine phosphoribosyltransferase -
AN206_RS20260 3964625..3966082 + 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
AN206_RS20265 3966139..3966753 - 615 WP_000602123.1 peptide chain release factor H -
AN206_RS20270 3966750..3967889 - 1140 WP_000521577.1 RNA ligase RtcB family protein -
AN206_RS20275 3968079..3968531 - 453 WP_001059895.1 GNAT family N-acetyltransferase -
AN206_RS20280 3968541..3968939 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
AN206_RS20285 3968942..3969235 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
AN206_RS20290 3969287..3970342 - 1056 WP_001226168.1 DNA polymerase IV -
AN206_RS20295 3970413..3971198 - 786 WP_000207544.1 putative lateral flagellar export/assembly protein LafU -
AN206_RS20300 3971170..3972882 + 1713 Protein_3899 flagellar biosynthesis protein FlhA -
AN206_RS30800 3972961..3973119 + 159 WP_014639450.1 hypothetical protein -
AN206_RS20305 3973202..3973699 - 498 WP_000006241.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T56806 WP_001263500.1 NZ_CP012625:c3968939-3968541 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T56806 NZ_CP012625:c3968939-3968541 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT56806 WP_000554758.1 NZ_CP012625:c3969235-3968942 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT56806 NZ_CP012625:c3969235-3968942 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References