Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2600833..2601395 Replicon chromosome
Accession NZ_CP012625
Organism Escherichia coli strain SF-468

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag AN206_RS13150 Protein ID WP_000605675.1
Coordinates 2600833..2601111 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag AN206_RS13155 Protein ID WP_000781370.1
Coordinates 2601111..2601395 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AN206_RS13125 2596420..2596851 - 432 WP_000152310.1 peroxiredoxin OsmC -
AN206_RS13130 2597196..2597411 + 216 WP_000495766.1 biofilm-dependent modulation protein -
AN206_RS13135 2597513..2597650 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
AN206_RS13140 2597806..2599503 + 1698 WP_000433462.1 malate dehydrogenase -
AN206_RS13145 2599637..2600647 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
AN206_RS13150 2600833..2601111 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AN206_RS13155 2601111..2601395 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
AN206_RS13160 2601624..2602277 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
AN206_RS13165 2602270..2603154 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
AN206_RS13170 2603167..2606214 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T56803 WP_000605675.1 NZ_CP012625:2600833-2601111 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T56803 NZ_CP012625:2600833-2601111 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT56803 WP_000781370.1 NZ_CP012625:2601111-2601395 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT56803 NZ_CP012625:2601111-2601395 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References