Detailed information of TA system TA056792

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2055295..2055797
• Replicon: chromosome
• Accession: NZ_CP012625
• Organism: Escherichia coli strain SF-468

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: AN206_RS10320
• Protein ID: WP_000767819.1
• Coordinates: 2055543..2055797 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: AN206_RS10315
• Protein ID: WP_001259253.1
• Coordinates: 2055295..2055546 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AN206_RS10295	2050473..2051540	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
AN206_RS10300	2051540..2052610	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
AN206_RS10305	2052607..2053911	-	1305	WP_001296211.1	histidinol dehydrogenase	-
AN206_RS10310	2053917..2054816	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
AN206_RS27910	2054962..2055012	-	51	WP_001364200.1	his operon leader peptide	-
AN206_RS10315	2055295..2055546	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
AN206_RS10320	2055543..2055797	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
AN206_RS10325	2055880..2056704	+	825	WP_000754737.1	SDR family oxidoreductase	-
AN206_RS10330	2056750..2057679	+	930	WP_000803366.1	LysR family transcriptional regulator	-
AN206_RS30295	2057894..2057956	+	63	WP_010723108.1	membrane protein YoeI	-
AN206_RS10335	2057946..2059304	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
AN206_RS10345	2059521..2059979	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2059521..2059979	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T56792 WP_000767819.1 NZ_CP012625:2055543-2055797 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T56792 NZ_CP012625:2055543-2055797 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT56792 WP_001259253.1 NZ_CP012625:2055295-2055546 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT56792 NZ_CP012625:2055295-2055546 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3