Detailed information of TA system TA056754

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-couple_hipB
• Location: 482075..482723
• Replicon: chromosome
• Accession: NZ_CP012588
• Organism: Streptococcus thermophilus strain MN-BM-A01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AMD33_RS02510
• Protein ID: WP_014727406.1
• Coordinates: 482075..482440 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: J7TXL7
• Locus tag: AMD33_RS02515
• Protein ID: WP_002890617.1
• Coordinates: 482430..482723 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMD33_RS02495	478583..479728	+	1146	WP_014727403.1	abortive infection protein	-
AMD33_RS02500	479889..480608	+	720	WP_014727404.1	hypothetical protein	-
AMD33_RS10895	480660..481910	+	1251	WP_014727405.1	restriction endonuclease subunit S	-
AMD33_RS02510	482075..482440	+	366	WP_014727406.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AMD33_RS02515	482430..482723	+	294	WP_002890617.1	helix-turn-helix transcriptional regulator	Antitoxin
AMD33_RS02520	482745..484346	+	1602	WP_041829288.1	type I restriction-modification system subunit M	-
AMD33_RS02525	484424..484843	+	420	Protein_485	PD-(D/E)XK nuclease family transposase	-
AMD33_RS02530	484944..485894	+	951	Protein_486	DNA/RNA non-specific endonuclease	-
AMD33_RS02535	486094..486654	+	561	WP_014621411.1	LemA family protein	-
AMD33_RS02540	486656..487555	+	900	WP_014621412.1	zinc metalloprotease HtpX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	474543..482723	8180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-88)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14511.65 Da
• Isoelectric Point: 9.3397

>T56754 WP_014727406.1 NZ_CP012588:482075-482440 [Streptococcus thermophilus]
VHAIYFYKDKQGNQPVLDYMRELARHDSKDSRIKLNKLNDYIELLSQHGTCAGQPYIKHLDAEIWELRPLRDRILFVAWL
DGSFVLLHHFVKKTQKTPRREIEKAKRELQDLKERGLRDEE

Nucleotide

• Length: 366 bp

>T56754 NZ_CP012588:482075-482440 [Streptococcus thermophilus]
GTGCACGCTATTTATTTTTATAAGGACAAACAGGGGAATCAACCGGTCTTGGACTACATGAGAGAGCTAGCTCGTCACGA
TAGCAAGGACAGCCGGATCAAACTCAACAAGCTCAACGACTACATCGAACTTTTGAGCCAACATGGTACCTGTGCTGGCC
AACCCTATATCAAGCATCTGGATGCGGAGATTTGGGAGTTGAGACCCTTGCGGGATCGGATTTTATTCGTGGCTTGGTTG
GATGGAAGCTTTGTCTTACTGCATCATTTTGTCAAAAAGACGCAAAAGACACCTAGACGAGAGATCGAGAAAGCCAAGCG
GGAGTTGCAAGATTTGAAGGAAAGAGGGCTAAGAGATGAAGAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10778.52 Da
• Isoelectric Point: 5.8962

>AT56754 WP_002890617.1 NZ_CP012588:482430-482723 [Streptococcus thermophilus]
MKNSAIGSNWKDVRAELFTKEEILESDLRVAIMTELIEARHEKGFSQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEQEKL

Nucleotide

• Length: 294 bp

>AT56754 NZ_CP012588:482430-482723 [Streptococcus thermophilus]
ATGAAGAATAGTGCCATCGGCAGCAATTGGAAAGATGTGAGAGCAGAGCTCTTCACAAAGGAAGAAATCCTGGAAAGTGA
TCTGCGGGTCGCGATTATGACGGAGCTAATCGAGGCTCGGCATGAAAAGGGCTTTAGCCAAAAGAAACTAGAAGAACTCA
GTGGGGTCAGTCAACCGGTCATCGCTCGGATGGAGACGGGAAAGACCAGTCCTCAATTGGACACGGTCTTGAAAGTCTTA
GCCAGTCTGGGCAAGACCCTTGCTGTCGTCCCACTGGAACAAGAAAAGCTCTAA

Structures

Antitoxin

• AlphaFold DB: J7TXL7