Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5574871..5575466 Replicon chromosome
Accession NZ_CP012583
Organism Pseudomonas aeruginosa strain PA_D22

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag A6701_RS31790 Protein ID WP_003113526.1
Coordinates 5575188..5575466 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag A6701_RS25655 Protein ID WP_003133769.1
Coordinates 5574871..5575176 (-) Length 102 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6701_RS25620 5570012..5570860 + 849 WP_069376896.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
A6701_RS25630 5571027..5571968 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
A6701_RS25635 5572085..5572699 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
A6701_RS25640 5572741..5573325 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
A6701_RS25645 5573366..5574466 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
A6701_RS25655 5574871..5575176 - 306 WP_003133769.1 HigA family addiction module antidote protein Antitoxin
A6701_RS31790 5575188..5575466 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A6701_RS25660 5575795..5578023 + 2229 WP_033938414.1 TonB-dependent receptor -
A6701_RS25665 5578093..5578740 - 648 WP_003095021.1 carbonate dehydratase -
A6701_RS25670 5578802..5580040 - 1239 WP_003095023.1 C69 family dipeptidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-92)

Antitoxin

(6-82)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T56740 WP_003113526.1 NZ_CP012583:c5575466-5575188 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T56740 NZ_CP012583:c5575466-5575188 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11214.73 Da        Isoelectric Point: 4.6081

>AT56740 WP_003133769.1 NZ_CP012583:c5575176-5574871 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Download         Length: 306 bp

>AT56740 NZ_CP012583:c5575176-5574871 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V6ALY3


Antitoxin

Source ID Structure

References