Detailed information of TA system TA056722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5538367..5538962
• Replicon: chromosome
• Accession: NZ_CP012580
• Organism: Pseudomonas aeruginosa strain PA_D9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: A6688_RS31560
• Protein ID: WP_003113526.1
• Coordinates: 5538684..5538962 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A6688_RS25505
• Protein ID: WP_003133769.1
• Coordinates: 5538367..5538672 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A6688_RS25470	5533508..5534356	+	849	WP_069376896.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
A6688_RS25480	5534523..5535464	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
A6688_RS25485	5535581..5536195	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
A6688_RS25490	5536237..5536821	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
A6688_RS25495	5536862..5537962	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
A6688_RS25505	5538367..5538672	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
A6688_RS31560	5538684..5538962	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A6688_RS25510	5539291..5541519	+	2229	WP_033938414.1	TonB-dependent receptor	-
A6688_RS25515	5541589..5542236	-	648	WP_003095021.1	carbonate dehydratase	-
A6688_RS25520	5542298..5543536	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T56722 WP_003113526.1 NZ_CP012580:c5538962-5538684 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T56722 NZ_CP012580:c5538962-5538684 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT56722 WP_003133769.1 NZ_CP012580:c5538672-5538367 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT56722 NZ_CP012580:c5538672-5538367 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3