Detailed information of TA system TA056716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5574882..5575477
• Replicon: chromosome
• Accession: NZ_CP012579
• Organism: Pseudomonas aeruginosa strain PA_D5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: A7331_RS31750
• Protein ID: WP_003113526.1
• Coordinates: 5575199..5575477 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7331_RS25625
• Protein ID: WP_003133769.1
• Coordinates: 5574882..5575187 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7331_RS25590	5570023..5570871	+	849	WP_069376896.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
A7331_RS25600	5571038..5571979	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
A7331_RS25605	5572096..5572710	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
A7331_RS25610	5572752..5573336	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
A7331_RS25615	5573377..5574477	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
A7331_RS25625	5574882..5575187	-	306	WP_003133769.1	HigA family addiction module antidote protein	Antitoxin
A7331_RS31750	5575199..5575477	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A7331_RS25630	5575806..5578034	+	2229	WP_033938414.1	TonB-dependent receptor	-
A7331_RS25635	5578104..5578751	-	648	WP_003095021.1	carbonate dehydratase	-
A7331_RS25640	5578813..5580051	-	1239	WP_003095023.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T56716 WP_003113526.1 NZ_CP012579:c5575477-5575199 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T56716 NZ_CP012579:c5575477-5575199 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT56716 WP_003133769.1 NZ_CP012579:c5575187-5574882 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT56716 NZ_CP012579:c5575187-5574882 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3