56710

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5535886..5536481 Replicon chromosome
Accession NZ_CP012578
Organism Pseudomonas aeruginosa strain PA_D2

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag A6681_RS31520 Protein ID WP_003113526.1
Coordinates 5536203..5536481 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag A6681_RS25485 Protein ID WP_003133769.1
Coordinates 5535886..5536191 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A6681_RS25450 5531027..5531875 + 849 WP_069376896.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
A6681_RS25460 5532042..5532983 + 942 WP_003099281.1 ribose-phosphate pyrophosphokinase -
A6681_RS25465 5533100..5533714 + 615 WP_003095013.1 50S ribosomal protein L25/general stress protein Ctc -
A6681_RS25470 5533756..5534340 + 585 WP_003099278.1 aminoacyl-tRNA hydrolase -
A6681_RS25475 5534381..5535481 + 1101 WP_003099270.1 redox-regulated ATPase YchF -
A6681_RS25485 5535886..5536191 - 306 WP_003133769.1 HigA family addiction module antidote protein Antitoxin
A6681_RS31520 5536203..5536481 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
A6681_RS25490 5536810..5539038 + 2229 WP_033938414.1 TonB-dependent receptor -
A6681_RS25495 5539108..5539755 - 648 WP_003095021.1 carbonate dehydratase -
A6681_RS25500 5539817..5541055 - 1239 WP_003095023.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T56710 WP_003113526.1 NZ_CP012578:c5536481-5536203 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T56710 NZ_CP012578:c5536481-5536203 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGCTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11214.73 Da        Isoelectric Point: 4.6081

>AT56710 WP_003133769.1 NZ_CP012578:c5536191-5535886 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Download         Length: 306 bp

>AT56710 NZ_CP012578:c5536191-5535886 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTACTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References