Detailed information of TA system TA056664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1277292..1277932
• Replicon: chromosome
• Accession: NZ_CP012561
• Organism: Klebsiella pneumoniae strain UCLAOXA232KP

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W9BBY1
• Locus tag: AM277_RS06425
• Protein ID: WP_016529833.1
• Coordinates: 1277292..1277639 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM277_RS06430
• Protein ID: WP_040181955.1
• Coordinates: 1277639..1277932 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM277_RS06415	1273218..1274651	+	1434	WP_002920564.1	glycogen synthase GlgA	-
AM277_RS06420	1274669..1277116	+	2448	WP_002920561.1	glycogen phosphorylase	-
AM277_RS06425	1277292..1277639	+	348	WP_016529833.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM277_RS06430	1277639..1277932	+	294	WP_040181955.1	helix-turn-helix transcriptional regulator	Antitoxin
AM277_RS06435	1278002..1279510	-	1509	WP_023301456.1	glycerol-3-phosphate dehydrogenase	-
AM277_RS06440	1279715..1280044	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
AM277_RS06445	1280095..1280925	+	831	WP_024622927.1	rhomboid family intramembrane serine protease GlpG	-
AM277_RS06450	1280975..1281733	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13549.56 Da
• Isoelectric Point: 5.6749

>T56664 WP_016529833.1 NZ_CP012561:1277292-1277639 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T56664 NZ_CP012561:1277292-1277639 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10539.30 Da
• Isoelectric Point: 10.1464

>AT56664 WP_040181955.1 NZ_CP012561:1277639-1277932 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKSLV

Nucleotide

• Length: 294 bp

>AT56664 NZ_CP012561:1277639-1277932 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAAGTCTCGTTTAA

Structures

Toxin

• AlphaFold DB: W9BBY1