Detailed information of TA system TA056631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3220705..3221369
• Replicon: chromosome
• Accession: NZ_CP012554
• Organism: Citrobacter portucalensis strain P10159

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: P10159_RS15350
• Protein ID: WP_032942116.1
• Coordinates: 3220705..3221007 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5A9BY49
• Locus tag: P10159_RS15355
• Protein ID: WP_008786950.1
• Coordinates: 3221028..3221369 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P10159_RS15335	3216248..3217771	-	1524	WP_060683103.1	sugar ABC transporter ATP-binding protein	-
P10159_RS15340	3217886..3220150	+	2265	WP_060683104.1	hybrid sensor histidine kinase/response regulator	-
P10159_RS15345	3220220..3220549	+	330	WP_003031807.1	hypothetical protein	-
P10159_RS15350	3220705..3221007	+	303	WP_032942116.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P10159_RS15355	3221028..3221369	+	342	WP_008786950.1	HigA family addiction module antidote protein	Antitoxin
P10159_RS15360	3221422..3222180	-	759	WP_060683105.1	phosphonate metabolism protein PhnP	-
P10159_RS15365	3222189..3222623	-	435	WP_008786952.1	aminoalkylphosphonate N-acetyltransferase	-
P10159_RS15370	3222610..3223164	-	555	WP_008786953.1	ribose 1,5-bisphosphokinase	-
P10159_RS15375	3223167..3224303	-	1137	WP_060683106.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
P10159_RS15380	3224300..3224986	-	687	WP_003826708.1	phosphonate C-P lyase system protein PhnL	-
P10159_RS15385	3225157..3225915	-	759	WP_060683107.1	phosphonate C-P lyase system protein PhnK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11860.49 Da
• Isoelectric Point: 9.9581

>T56631 WP_032942116.1 NZ_CP012554:3220705-3221007 [Citrobacter portucalensis]
MSKSLNIRNFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWINGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T56631 NZ_CP012554:3220705-3221007 [Citrobacter portucalensis]
ATGTCTAAATCATTAAATATCAGAAATTTTCGGGATACATGGCTTGAAGATTTTTTTGAACGGGCGACATCGCACAGAAA
AATACCTGCGGATATTCACACTGCATTAGCCAGAAAGCTGGATATCATTAATGCGGCTGTTTCGCATCGGGATTTGCGCT
CGCCACCCGGTAACAGATATGAAGAGTTAACCGGAAAATTACAGGAATATTCTTCAATCAGAGTAAACAAGCAGTACCGA
TTAATATTTAAATGGATTAATGGTAAGGCCGAAGATGTGTACTTAGATCCACACATCTATTAG

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12986.83 Da
• Isoelectric Point: 6.4743

>AT56631 WP_008786950.1 NZ_CP012554:3221028-3221369 [Citrobacter portucalensis]
MKQASRKPTTVGDILQYEYLEPLDLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRDVGAKKVA

Nucleotide

• Length: 342 bp

>AT56631 NZ_CP012554:3221028-3221369 [Citrobacter portucalensis]
ATGAAACAGGCATCCAGAAAACCAACGACTGTGGGTGATATCCTGCAATATGAATACCTGGAACCGCTTGATCTGAAAAT
CAACGATCTGGCTGAAATTCTGCATGTTCACCGCAATACAGTGAGCGCACTGGTCAACAATAATCGCAAATTAACAACGG
ATATGGCGTTTCGTTTGGCAAAAGCGTTCGATACCTCCGTTGATTTCTGGCGTAACCTACAGGCGGCGGTTGACCTGTGG
GAAGTTGAAAATGACATGCGGGCGCAAGAAGAACTGAACCGGATTGTTTCAGTTAAAGATTTTATTGCGCAGCGAGATGT
CGGCGCAAAAAAAGTCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5A9BY49