Detailed information of TA system TA056629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2975669..2976268
• Replicon: chromosome
• Accession: NZ_CP012554
• Organism: Citrobacter portucalensis strain P10159

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: P10159_RS14200
• Protein ID: WP_060683044.1
• Coordinates: 2975669..2975980 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: P10159_RS14205
• Protein ID: WP_038635893.1
• Coordinates: 2975981..2976268 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P10159_RS14165	2970778..2971377	+	600	WP_060683041.1	glucose-1-phosphatase	-
P10159_RS14170	2971371..2972243	+	873	WP_008786998.1	virulence factor BrkB family protein	-
P10159_RS14175	2972240..2972677	+	438	WP_003825289.1	D-tyrosyl-tRNA(Tyr) deacylase	-
P10159_RS14180	2972722..2973663	+	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
P10159_RS14185	2973681..2974127	-	447	WP_060683042.1	helix-turn-helix domain-containing protein	-
P10159_RS14190	2974124..2974435	-	312	WP_048215202.1	type II toxin-antitoxin system HigB family toxin	-
P10159_RS14195	2974520..2975449	-	930	WP_060683043.1	alpha/beta hydrolase	-
P10159_RS14200	2975669..2975980	+	312	WP_060683044.1	hypothetical protein	Toxin
P10159_RS14205	2975981..2976268	+	288	WP_038635893.1	helix-turn-helix domain-containing protein	Antitoxin
P10159_RS14210	2976312..2977241	-	930	WP_060683045.1	formate dehydrogenase accessory protein FdhE	-
P10159_RS14215	2977238..2977873	-	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
P10159_RS14220	2977870..2978772	-	903	WP_003825273.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-74)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12374.54 Da
• Isoelectric Point: 10.2893

>T56629 WP_060683044.1 NZ_CP012554:2975669-2975980 [Citrobacter portucalensis]
MLFIETEIFTEDVKKLLDDDEYRRLQIFLAIQPDCGDLIQETGGLRKVRWRARGKGKRSGVRIIYFHQIRKSQIRLLLIY
QKGIKDDLTPQEKVLLRMLNERW

Nucleotide

• Length: 312 bp

>T56629 NZ_CP012554:2975669-2975980 [Citrobacter portucalensis]
ATGCTATTCATAGAAACGGAAATCTTCACTGAAGACGTCAAAAAATTACTGGATGATGATGAATACCGTAGATTACAGAT
ATTCCTTGCGATTCAGCCTGATTGTGGCGATCTGATACAAGAAACGGGCGGACTGAGAAAGGTTCGCTGGAGAGCGCGAG
GGAAAGGCAAACGTAGCGGTGTGAGGATTATTTACTTTCATCAAATCAGAAAATCACAAATTCGATTGTTGCTGATTTAT
CAGAAAGGGATCAAGGATGATCTGACTCCACAAGAAAAAGTGCTGCTGCGTATGTTGAATGAGAGGTGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10761.36 Da
• Isoelectric Point: 11.1842

>AT56629 WP_038635893.1 NZ_CP012554:2975981-2976268 [Citrobacter portucalensis]
MDKALFERLTQSMVQMNEIAEGTRQPSRTFHIDAMKIKEIRQSSGLSQTRFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPKHVIQALR

Nucleotide

• Length: 288 bp

>AT56629 NZ_CP012554:2975981-2976268 [Citrobacter portucalensis]
ATGGATAAGGCACTATTTGAGCGATTAACTCAAAGTATGGTTCAGATGAATGAGATCGCTGAAGGAACGCGACAACCTTC
CCGTACCTTTCATATTGACGCCATGAAAATAAAGGAAATTCGCCAGTCTTCAGGATTATCACAGACTCGTTTCGCAGATT
TGATATCGGTAAGCGTGGATACGTTGCGAAATTGGGAGCAAGGGCGTCGTTCACCGACCGGTCCAGCAAAAGCGTTGTTA
CGTGCTATTGCCAATGATCCAAAACATGTCATTCAGGCACTAAGATAA