Detailed information of TA system TA056623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1690422..1690927
• Replicon: chromosome
• Accession: NZ_CP012549
• Organism: Campylobacter sp. RM16187

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CDOMF_RS08985
• Protein ID: WP_260951642.1
• Coordinates: 1690703..1690927 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CDOMF_RS08980
• Protein ID: WP_260951641.1
• Coordinates: 1690422..1690706 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CDOMF_RS08960 (CDOMF_1762)	1685824..1687005	-	1182	WP_260951637.1	AAA family ATPase	-
CDOMF_RS08965 (CDOMF_1763)	1687812..1688531	-	720	WP_260951638.1	hypothetical protein	-
CDOMF_RS08970 (CDOMF_1764)	1688519..1689466	-	948	WP_260951639.1	hypothetical protein	-
CDOMF_RS08975 (CDOMF_1765)	1689478..1690410	-	933	WP_260951640.1	patatin-like phospholipase family protein	-
CDOMF_RS08980 (CDOMF_1766)	1690422..1690706	-	285	WP_260951641.1	helix-turn-helix transcriptional regulator	Antitoxin
CDOMF_RS08985 (CDOMF_1767)	1690703..1690927	-	225	WP_260951642.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CDOMF_RS08990	1691862..1692005	+	144	WP_260951643.1	integrase arm-type DNA-binding domain-containing protein	-
CDOMF_RS08995 (CDOMF_1768)	1691995..1693104	+	1110	WP_260951644.1	tyrosine-type recombinase/integrase	-
CDOMF_RS09000 (CDOMF_1769)	1693115..1693903	+	789	WP_260951645.1	hypothetical protein	-
CDOMF_RS09005 (CDOMF_1770)	1694045..1694233	+	189	WP_170020669.1	helix-turn-helix domain-containing protein	-
CDOMF_RS09010 (CDOMF_1771)	1694343..1694684	+	342	WP_260951646.1	hypothetical protein	-
CDOMF_RS09015 (CDOMF_1772)	1694732..1694917	+	186	WP_260951647.1	hypothetical protein	-
CDOMF_RS09020	1694907..1695035	+	129	WP_260951648.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1670203..1691419	21216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (2-71)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8152.79 Da
• Isoelectric Point: 11.1343

>T56623 WP_260951642.1 NZ_CP012549:c1690927-1690703 [Campylobacter sp. RM16187]
MAKEVGLNLGIPHSAPLGNGLFEFRTKGKGGIARSVFVNVINREIAILHTLIKKSDAIPKKDMKIIMQRAKEIK

Nucleotide

• Length: 225 bp

>T56623 NZ_CP012549:c1690927-1690703 [Campylobacter sp. RM16187]
ATGGCAAAAGAGGTTGGACTAAATTTGGGCATACCACATAGTGCTCCTCTTGGAAACGGGCTATTTGAGTTTAGGACAAA
AGGCAAAGGAGGTATTGCAAGAAGTGTCTTTGTAAACGTCATAAATAGAGAAATAGCGATTTTGCATACGTTGATTAAAA
AATCAGACGCTATCCCTAAAAAAGATATGAAGATCATTATGCAAAGAGCAAAGGAGATAAAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10929.61 Da
• Isoelectric Point: 10.3716

>AT56623 WP_260951641.1 NZ_CP012549:c1690706-1690422 [Campylobacter sp. RM16187]
MRPTFEDFKEKALKRSEVKKEYDRLEIEFELKLKLIKIRKAANLTQEEMASRMNTSKSNISRLESLNSKISPTISTLNAY
ANAAGYRLDVNFVR

Nucleotide

• Length: 285 bp

>AT56623 NZ_CP012549:c1690706-1690422 [Campylobacter sp. RM16187]
ATGAGACCAACATTTGAGGACTTTAAAGAAAAAGCTCTAAAAAGAAGCGAGGTAAAAAAAGAATACGATAGGCTGGAGAT
TGAGTTTGAGTTAAAACTAAAGCTTATAAAGATTAGAAAAGCCGCAAATTTGACGCAAGAGGAGATGGCTAGTAGAATGA
ATACTAGTAAAAGCAACATCTCAAGATTAGAGAGCCTAAACTCTAAAATTTCACCAACAATCTCTACTTTAAATGCCTAT
GCAAATGCTGCAGGATATAGGCTTGATGTAAATTTTGTTCGTTAG