Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 142306..142842 Replicon plasmid pPSB1-3
Accession NZ_CP012511
Organism Piscirickettsia salmonis strain PM32597B1

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1L6THY5
Locus tag KU39_RS17145 Protein ID WP_036772441.1
Coordinates 142570..142842 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag KU39_RS17140 Protein ID WP_032126795.1
Coordinates 142306..142566 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU39_RS17110 138122..138811 + 690 WP_017375909.1 hypothetical protein -
KU39_RS17115 138859..139674 + 816 WP_036775032.1 hypothetical protein -
KU39_RS19240 139758..139964 + 207 Protein_179 IS6 family transposase -
KU39_RS20295 140015..140182 - 168 Protein_180 IS6 family transposase -
KU39_RS17125 140252..140884 + 633 Protein_181 IS6-like element ISPsa2 family transposase -
KU39_RS17130 140950..141516 - 567 Protein_182 IS6 family transposase -
KU39_RS20305 141802..141966 - 165 Protein_183 IS6 family transposase -
KU39_RS17140 142306..142566 + 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KU39_RS17145 142570..142842 + 273 WP_036772441.1 Txe/YoeB family addiction module toxin Toxin
KU39_RS17150 142918..143646 + 729 WP_017375910.1 IS6 family transposase -
KU39_RS17155 144216..145280 - 1065 WP_174872554.1 IS91 family transposase -
KU39_RS21145 145460..145639 - 180 WP_157894752.1 hypothetical protein -
KU39_RS17165 146052..146879 + 828 WP_048876208.1 IS30 family transposase -
KU39_RS17170 146922..147651 - 730 Protein_190 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..181035 181035
- inside IScluster/Tn - - 136259..153265 17006


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10785.31 Da        Isoelectric Point: 7.8926

>T56603 WP_036772441.1 NZ_CP012511:142570-142842 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Download         Length: 273 bp

>T56603 NZ_CP012511:142570-142842 [Piscirickettsia salmonis]
TTGTCAAAACTAAACTTACTTACTTGGAGTGATGATGCTTGGGATGATTACCTTTATTGGCAAGATACAGATAAAAAGGT
GCTTAAAAAAATCAATAAACTTATAAAGGAATGTCAACGCATGCCGACAGAAGGTATTGGTCATCCCGAACCTTTAAAAC
ATGATTTCAGTGGCTATTGGAGCCGAAAAATAAAGGGTGAGCATAGATTAGTTTATTCATTTGATGCGACCAGTTTAATG
ATTTCTGCATGCCGTTACCATTATGATCAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT56603 WP_032126795.1 NZ_CP012511:142306-142566 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT56603 NZ_CP012511:142306-142566 [Piscirickettsia salmonis]
ATGCTACGAGATAACATCAATGCTACGGCAGCACGGGATAATTTTAAGAGTACCTGTGATACAGTGTGTGAAAACAATGC
TCCTGTACTTGTGAAGCGCAAGGGTGCTGAGGATGTCGTTATTATTTCTGCTTCTGACTTTGGTGGTATGGAAGAAACGA
TCCACTTATTAAGTAGTAGAATAATGGCAACACGCCTTATGAGAAGTATGAAACAACTTGATGAAGGTAAAGGTGTTCAA
CGTGATATTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6THY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References