Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/-
Location 2971609..2972257 Replicon chromosome
Accession NZ_CP012506
Organism Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID P9WJ12
Locus tag AFL40_RS14005 Protein ID WP_003899414.1
Coordinates 2971609..2971932 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ10
Locus tag AFL40_RS14010 Protein ID WP_003899415.1
Coordinates 2972012..2972257 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFL40_RS13985 2967183..2968444 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
AFL40_RS13990 2968818..2970257 - 1440 WP_003901443.1 phage major capsid protein -
AFL40_RS13995 2970265..2970798 - 534 WP_003899412.1 HK97 family phage prohead protease -
AFL40_RS21550 2970951..2971442 - 492 WP_003900541.1 phage terminase small subunit P27 family -
AFL40_RS14005 2971609..2971932 - 324 WP_003899414.1 type II toxin-antitoxin system toxin Toxin
AFL40_RS14010 2972012..2972257 - 246 WP_003899415.1 type II toxin-antitoxin system antitoxin Antitoxin
AFL40_RS14015 2972254..2973681 - 1428 WP_003899416.1 DUF3631 domain-containing protein -
AFL40_RS14020 2973683..2974075 - 393 WP_003899417.1 DUF2742 domain-containing protein -
AFL40_RS14025 2974072..2974332 - 261 WP_003899418.1 helix-turn-helix domain-containing protein -
AFL40_RS14030 2974349..2974711 - 363 WP_003900543.1 hypothetical protein -
AFL40_RS14035 2974714..2975841 - 1128 WP_003899420.1 site-specific integrase -
AFL40_RS14040 2975986..2976213 - 228 WP_003899421.1 hypothetical protein -
AFL40_RS21555 2976210..2976599 - 390 WP_003899422.1 hypothetical protein -
AFL40_RS14045 2976505..2976777 + 273 WP_003900544.1 hypothetical protein -
AFL40_RS14050 2976876..2977109 + 234 WP_003413717.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2965574..2975841 10267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-82)

Antitoxin

Cro/C1-type helix-turn-helix domain

(71-122)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12359.82 Da        Isoelectric Point: 8.6717

>T56586 WP_003899414.1 NZ_CP012506:c2971932-2971609 [Mycobacterium tuberculosis]
MTHKRTKRQPAIAAGLNAPRRNRVGRQHGWPADVPSAEQRRAQRQRDLEAIRRAYAEMVATSHEIDDDTAELALLSMHLD
DEQRRLEAGMKLGWHPYHFPDEPDSKQ

Download         Length: 324 bp

>T56586 NZ_CP012506:c2971932-2971609 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 7697.71 Da        Isoelectric Point: 4.8565

>AT56586 WP_003899415.1 NZ_CP012506:c2972257-2972012 [Mycobacterium tuberculosis]
MSGHALAARTLLAAADELVGGPPVEASAAALAGDAAGAWRTAAVELARALVRAVAESHGVAAVLFAATAAAAAAVDRGDP
P

Download         Length: 246 bp

>AT56586 NZ_CP012506:c2972257-2972012 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A045IHC4

Antitoxin

Source ID Structure
AlphaFold DB A0A806JR81

References