Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Gp49-HTH_37
Location 2186717..2187585 Replicon chromosome
Accession NZ_CP012506
Organism Mycobacterium tuberculosis strain SCAID 187.0

Toxin (Protein)


Gene name higB Uniprot ID P9WJA4
Locus tag AFL40_RS10165 Protein ID WP_010886136.1
Coordinates 2186717..2187094 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0TLM0
Locus tag AFL40_RS10170 Protein ID WP_003409886.1
Coordinates 2187136..2187585 (+) Length 150 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AFL40_RS10115 2182506..2182958 - 453 WP_031664647.1 hypothetical protein -
AFL40_RS10120 2183022..2183423 + 402 WP_003409869.1 hypothetical protein -
AFL40_RS10125 2183416..2183598 - 183 WP_003409870.1 hypothetical protein -
AFL40_RS10130 2183712..2184062 - 351 WP_003409871.1 hypothetical protein -
AFL40_RS10135 2184073..2184975 - 903 WP_003899097.1 hypothetical protein -
AFL40_RS10140 2184996..2185187 - 192 WP_003409876.1 hypothetical protein -
AFL40_RS10145 2185188..2185484 - 297 WP_003409877.1 hypothetical protein -
AFL40_RS10150 2185724..2185939 + 216 WP_003409878.1 antitoxin -
AFL40_RS10155 2185936..2186247 + 312 WP_003409881.1 type II toxin-antitoxin system VapC family toxin -
AFL40_RS22555 2186221..2186742 - 522 WP_003904745.1 hypothetical protein -
AFL40_RS10165 2186717..2187094 + 378 WP_010886136.1 type II toxin-antitoxin system toxin HigB Toxin
AFL40_RS10170 2187136..2187585 + 450 WP_003409886.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
AFL40_RS10175 2187582..2188127 + 546 WP_003409891.1 SecB-like chaperone -
AFL40_RS10180 2188016..2188630 - 615 WP_003901296.1 hypothetical protein -
AFL40_RS10185 2188679..2188975 - 297 WP_003409896.1 type II toxin-antitoxin system RelE/ParE family toxin -
AFL40_RS10190 2188972..2189223 - 252 WP_003409899.1 type II toxin-antitoxin system ParD family antitoxin -
AFL40_RS10195 2189210..2189704 + 495 WP_003899099.1 hypothetical protein -
AFL40_RS10200 2189864..2190271 - 408 WP_003409913.1 type II toxin-antitoxin system VapC family toxin -
AFL40_RS10205 2190275..2190547 - 273 WP_003899100.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
AFL40_RS10210 2190580..2191800 - 1221 WP_003409919.1 TetR family transcriptional regulator Mce3R -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-113)

Antitoxin

(44-91)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14397.77 Da        Isoelectric Point: 10.8862

>T56570 WP_010886136.1 NZ_CP012506:2186717-2187094 [Mycobacterium tuberculosis]
VPPPDPAAMGTWKFFRASVDGRPVFKKEFDKLPDQARAALIVLMQRYLVGDLAAGSIKPIRGDILELRWHEANNHFRVLF
FRWGQHPVALTAFYKNQQKTPKTKIETALDRQKIWKRAFGDTPPI

Download         Length: 378 bp

>T56570 NZ_CP012506:2186717-2187094 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 16760.16 Da        Isoelectric Point: 8.0771

>AT56570 WP_003409886.1 NZ_CP012506:2187136-2187585 [Mycobacterium tuberculosis]
MSIDFPLGDDLAGYIAEAIAADPSFKGTLEDAEEARRLVDALIALRKHCQLSQVEVAKRMGVRQPTVSGFEKEPSDPKLS
TLQRYARALDARLRLVLEVPTLREVPTWHRLSSYRGSARDHQVRVGADKEILMQTNWARHISVRQVEVA

Download         Length: 450 bp

>AT56570 NZ_CP012506:2187136-2187585 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCATCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 7AWK
PDB 7NBU


Antitoxin

Source ID Structure
PDB 5MTW
AlphaFold DB G0TLM0

References