Detailed information of TA system TA056532

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 195872..196141
• Replicon: plasmid pCFSAN004179G
• Accession: NZ_CP012501
• Organism: Escherichia coli strain 08-00022

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CP48_RS01165
• Protein ID: WP_001312861.1
• Coordinates: 195983..196141 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	195872..195937 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP48_RS31860	191058..191324	-	267	WP_187703271.1	hypothetical protein	-
CP48_RS31150	191394..191543	+	150	WP_024197809.1	hypothetical protein	-
CP48_RS01135	191626..192165	+	540	WP_047088942.1	single-stranded DNA-binding protein	-
CP48_RS01140	192223..192456	+	234	WP_000005990.1	DUF905 family protein	-
CP48_RS01145	192520..194484	+	1965	Protein_221	ParB/RepB/Spo0J family partition protein	-
CP48_RS01150	194553..194987	+	435	WP_000845944.1	conjugation system SOS inhibitor PsiB	-
CP48_RS01155	194984..195703	+	720	WP_000116354.1	plasmid SOS inhibition protein A	-
CP48_RS31865	195715..195903	-	189	WP_001355331.1	hypothetical protein	-
-	195715..195939	+	225	NuclAT_0	-	-
-	195715..195939	+	225	NuclAT_0	-	-
-	195715..195939	+	225	NuclAT_0	-	-
-	195715..195939	+	225	NuclAT_0	-	-
-	195872..195937	+	66	-	-	Antitoxin
CP48_RS01165	195983..196141	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CP48_RS31155	196589..196834	+	246	WP_187703272.1	hypothetical protein	-
CP48_RS01175	197190..198194	-	1005	WP_024166184.1	IS110 family transposase	-
CP48_RS01180	198445..198732	+	288	WP_000107542.1	hypothetical protein	-
CP48_RS31165	198851..199672	+	822	Protein_229	DUF945 domain-containing protein	-
CP48_RS30255	199970..200479	-	510	Protein_230	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / faeC / faeD / faeE / faeF / faeH / faeI / faeJ / stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC / estIa / estIa	1..242187	242187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T56532 WP_001312861.1 NZ_CP012501:195983-196141 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T56532 NZ_CP012501:195983-196141 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT56532 NZ_CP012501:195872-195937 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin