Detailed information of TA system TA056527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 20597..21168
• Replicon: plasmid pCFSAN004179G
• Accession: NZ_CP012501
• Organism: Escherichia coli strain 08-00022

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B0VD50
• Locus tag: CP48_RS00155
• Protein ID: WP_024166187.1
• Coordinates: 20597..20878 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: CP48_RS00160
• Protein ID: WP_001081196.1
• Coordinates: 20887..21168 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP48_RS00135	16335..17111	+	777	WP_000217412.1	transcriptional regulator LldR	-
CP48_RS00140	17108..18255	+	1148	Protein_24	quinone-dependent L-lactate dehydrogenase	-
CP48_RS31815	18920..19075	+	156	WP_000074142.1	hypothetical protein	-
CP48_RS00145	19421..19979	+	559	Protein_26	hypothetical protein	-
CP48_RS00150	19903..20457	+	555	Protein_27	IS21-like element ISEc10 family helper ATPase IstB	-
CP48_RS00155	20597..20878	+	282	WP_024166187.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CP48_RS00160	20887..21168	+	282	WP_001081196.1	HigA family addiction module antidote protein	Antitoxin
CP48_RS00165	21418..22072	-	655	Protein_30	helix-turn-helix domain-containing protein	-
CP48_RS00170	22225..23183	-	959	Protein_31	IS3 family transposase	-
CP48_RS00175	23232..24236	+	1005	WP_001179712.1	IS110 family transposase	-
CP48_RS00180	24504..24780	-	277	Protein_33	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / faeC / faeD / faeE / faeF / faeH / faeI / faeJ / stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC / estIa / estIa	1..242187	242187
	-	inside	IScluster/Tn	-	-	19702..41792	22090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10616.24 Da
• Isoelectric Point: 10.4126

>T56527 WP_024166187.1 NZ_CP012501:20597-20878 [Escherichia coli]
MIKSFRHKGLEHFFKTGSTAGIQAKHAVKLRIQLTALNMAKRPDDMSAPGWKLHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T56527 NZ_CP012501:20597-20878 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACACTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTAACCGCCCTTAATATGGCTAAACGCCCAGACGATATGTCTGCACCAGGCTGGAAATTAC
ACCCTCTGAAGGGAGCTGATCTTAAAGGCCATTGGGCTATATCTGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGTGAAGACGCAATTCTGGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10191.65 Da
• Isoelectric Point: 7.4026

>AT56527 WP_001081196.1 NZ_CP012501:20887-21168 [Escherichia coli]
MNRMHNPAHPGAVLREYLGEMSVTDAATALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TARPNIRPLFHHA

Nucleotide

• Length: 282 bp

>AT56527 NZ_CP012501:20887-21168 [Escherichia coli]
ATGAATCGTATGCATAATCCCGCGCACCCAGGCGCGGTTCTTCGTGAATATCTGGGAGAAATGTCAGTCACAGACGCTGC
CACCGCATTAGGCATTACTCGCACGGCGCTTTCCCGGATTCTGAATGAAAAAGCTGGTATCTCTGCGGATATGGCGCTCC
GCCTGGAATCCGCACTCGGAACTAGCGCCGAAATGTGGACTGGTATGCAATCTCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCGCTCGCCCAAACATTCGCCCATTATTTCACCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0B0VD50