Detailed information of TA system TA056523

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1733..1986
• Replicon: plasmid pCFSAN004179G
• Accession: NZ_CP012501
• Organism: Escherichia coli strain 08-00022

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CP48_RS00030
• Protein ID: WP_001312851.1
• Coordinates: 1837..1986 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	1733..1789 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP48_RS00010	74..403	-	330	WP_001137792.1	IS66 family insertion sequence hypothetical protein	-
CP48_RS00015	519..731	+	213	WP_001364091.1	hypothetical protein	-
CP48_RS00020	1058..1267	+	210	WP_001355319.1	hemolysin expression modulator Hha	-
CP48_RS31810	1305..1478	+	174	WP_157839839.1	hypothetical protein	-
-	1733..1789	-	57	NuclAT_1	-	Antitoxin
-	1733..1789	-	57	NuclAT_1	-	Antitoxin
-	1733..1789	-	57	NuclAT_1	-	Antitoxin
-	1733..1789	-	57	NuclAT_1	-	Antitoxin
CP48_RS00030	1837..1986	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CP48_RS00035	2270..2518	+	249	WP_000083838.1	replication regulatory protein RepA	-
CP48_RS00040	2762..2836	+	75	WP_001364037.1	RepA leader peptide Tap	-
CP48_RS00045	2829..3686	+	858	WP_000829162.1	incFII family plasmid replication initiator RepA	-
CP48_RS30100	4816..6186	-	1371	Protein_9	IS3 family transposase	-
CP48_RS00065	6232..6417	+	186	Protein_10	type II toxin-antitoxin system RelE/ParE family toxin	-
CP48_RS00070	6417..6704	+	288	WP_174498321.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	estIa / faeC / faeD / faeE / faeF / faeH / faeI / faeJ / stcE / exeE / exeG / hlyD / hlyB / hlyA / hlyC / estIa / estIa	1..242187	242187
	-	inside	IScluster/Tn	-	-	74..12996	12922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T56523 WP_001312851.1 NZ_CP012501:1837-1986 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T56523 NZ_CP012501:1837-1986 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCTGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT56523 NZ_CP012501:c1789-1733 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATCTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin