Detailed information of TA system TA056518

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 84196..84622
• Replicon: plasmid pCFSAN004180G
• Accession: NZ_CP012500
• Organism: Escherichia coli strain 09-00049

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GJ12_RS00505
• Protein ID: WP_001312861.1
• Coordinates: 84196..84354 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	84398..84622 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ12_RS00470	79360..80364	+	1005	WP_024166184.1	IS110 family transposase	-
GJ12_RS00490	81288..82859	-	1572	WP_065225491.1	IS66 family transposase	-
GJ12_RS00495	82879..83225	-	347	Protein_95	IS66 family insertion sequence element accessory protein TnpB	-
GJ12_RS00500	83225..83875	-	651	WP_040091510.1	IS66 family insertion sequence hypothetical protein	-
GJ12_RS00505	84196..84354	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	84398..84622	-	225	NuclAT_0	-	Antitoxin
-	84398..84622	-	225	NuclAT_0	-	Antitoxin
-	84398..84622	-	225	NuclAT_0	-	Antitoxin
-	84398..84622	-	225	NuclAT_0	-	Antitoxin
GJ12_RS30935	84434..84622	+	189	WP_001355331.1	hypothetical protein	-
GJ12_RS00515	84634..85353	-	720	WP_000116353.1	plasmid SOS inhibition protein A	-
GJ12_RS00520	85350..85784	-	435	WP_000845944.1	conjugation system SOS inhibitor PsiB	-
GJ12_RS00525	85853..87817	-	1965	WP_000117198.1	ParB/RepB/Spo0J family partition protein	-
GJ12_RS00530	87881..88114	-	234	WP_000005990.1	DUF905 family protein	-
GJ12_RS00535	88172..88624	-	453	WP_000290856.1	single-stranded DNA-binding protein	-
GJ12_RS28215	89266..89490	-	225	Protein_104	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / estIa / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..225292	225292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T56518 WP_001312861.1 NZ_CP012500:c84354-84196 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T56518 NZ_CP012500:c84354-84196 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT56518 NZ_CP012500:c84622-84398 [Escherichia coli]
TCACACGGATTGCCCGTGAACTGGCTGAACGACAAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGTAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin