Detailed information of TA system TA056513

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 38184..38437
• Replicon: plasmid pCFSAN004180G
• Accession: NZ_CP012500
• Organism: Escherichia coli strain 09-00049

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: GJ12_RS00235
• Protein ID: WP_001312851.1
• Coordinates: 38184..38333 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	38381..38437 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ12_RS00205	33700..34164	-	465	WP_040090730.1	hypothetical protein	-
GJ12_RS00210	34443..35483	-	1041	WP_136759863.1	hypothetical protein	-
GJ12_RS00220	36484..37341	-	858	WP_000829162.1	incFII family plasmid replication initiator RepA	-
GJ12_RS00225	37334..37408	-	75	WP_001364037.1	RepA leader peptide Tap	-
GJ12_RS00230	37652..37900	-	249	WP_000083838.1	replication regulatory protein RepA	-
GJ12_RS00235	38184..38333	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	38381..38437	+	57	NuclAT_1	-	Antitoxin
-	38381..38437	+	57	NuclAT_1	-	Antitoxin
-	38381..38437	+	57	NuclAT_1	-	Antitoxin
-	38381..38437	+	57	NuclAT_1	-	Antitoxin
GJ12_RS00240	38589..39053	-	465	WP_000455478.1	hypothetical protein	-
GJ12_RS00245	39208..39798	-	591	WP_000766788.1	DUF2726 domain-containing protein	-
GJ12_RS00250	39836..40045	-	210	WP_001355319.1	hemolysin expression modulator Hha	-
GJ12_RS00255	40373..40585	-	213	WP_001364091.1	hypothetical protein	-
GJ12_RS00260	40720..41280	-	561	WP_000139355.1	fertility inhibition protein FinO	-
GJ12_RS00265	41335..42072	-	738	WP_000429592.1	conjugal transfer pilus acetylase TraX	-
GJ12_RS00270	42094..42525	-	432	WP_000447372.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / estIa / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..225292	225292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T56513 WP_001312851.1 NZ_CP012500:c38333-38184 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T56513 NZ_CP012500:c38333-38184 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCTGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT56513 NZ_CP012500:38381-38437 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATCTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin