Detailed information of TA system TA056512

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 19040..19611
• Replicon: plasmid pCFSAN004180G
• Accession: NZ_CP012500
• Organism: Escherichia coli strain 09-00049

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B0VD50
• Locus tag: GJ12_RS00150
• Protein ID: WP_024166187.1
• Coordinates: 19330..19611 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: GJ12_RS00145
• Protein ID: WP_001081196.1
• Coordinates: 19040..19321 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ12_RS30145	14398..15092	+	695	WP_085948397.1	IS1 family transposase	-
GJ12_RS30915	15428..15704	+	277	Protein_20	transposase	-
GJ12_RS00130	15972..16976	-	1005	WP_001179712.1	IS110 family transposase	-
GJ12_RS00135	17025..17983	+	959	Protein_22	IS3 family transposase	-
GJ12_RS00140	18136..18790	+	655	Protein_23	helix-turn-helix domain-containing protein	-
GJ12_RS00145	19040..19321	-	282	WP_001081196.1	HigA family addiction module antidote protein	Antitoxin
GJ12_RS00150	19330..19611	-	282	WP_024166187.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJ12_RS00155	19751..20305	-	555	Protein_26	IS21-like element ISEc10 family helper ATPase IstB	-
GJ12_RS00160	20301..21083	-	783	Protein_27	hypothetical protein	-
GJ12_RS30920	21132..21287	-	156	WP_000074142.1	hypothetical protein	-
GJ12_RS00165	21895..22926	+	1032	WP_065224993.1	IS630 family transposase	-
GJ12_RS28160	22975..23181	+	207	WP_071961352.1	hypothetical protein	-
GJ12_RS31585	23189..23590	+	402	WP_052319587.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / estIa / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..225292	225292
	-	inside	IScluster/Tn	-	-	5..22926	22921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10616.24 Da
• Isoelectric Point: 10.4126

>T56512 WP_024166187.1 NZ_CP012500:c19611-19330 [Escherichia coli]
MIKSFRHKGLEHFFKTGSTAGIQAKHAVKLRIQLTALNMAKRPDDMSAPGWKLHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T56512 NZ_CP012500:c19611-19330 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACACTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTAACCGCCCTTAATATGGCTAAACGCCCAGACGATATGTCTGCACCAGGCTGGAAATTAC
ACCCTCTGAAGGGAGCTGATCTTAAAGGCCATTGGGCTATATCTGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGTGAAGACGCAATTCTGGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10191.65 Da
• Isoelectric Point: 7.4026

>AT56512 WP_001081196.1 NZ_CP012500:c19321-19040 [Escherichia coli]
MNRMHNPAHPGAVLREYLGEMSVTDAATALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TARPNIRPLFHHA

Nucleotide

• Length: 282 bp

>AT56512 NZ_CP012500:c19321-19040 [Escherichia coli]
ATGAATCGTATGCATAATCCCGCGCACCCAGGCGCGGTTCTTCGTGAATATCTGGGAGAAATGTCAGTCACAGACGCTGC
CACCGCATTAGGCATTACTCGCACGGCGCTTTCCCGGATTCTGAATGAAAAAGCTGGTATCTCTGCGGATATGGCGCTCC
GCCTGGAATCCGCACTCGGAACTAGCGCCGAAATGTGGACTGGTATGCAATCTCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCGCTCGCCCAAACATTCGCCCATTATTTCACCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0B0VD50