Detailed information of TA system TA056506

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 183922..184348
• Replicon: plasmid pCFSAN004181P
• Accession: NZ_CP012499
• Organism: Escherichia coli strain GB089

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CO57_RS00975
• Protein ID: WP_001312861.1
• Coordinates: 183922..184080 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	184124..184348 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO57_RS00940	179085..180089	+	1005	WP_024166184.1	IS110 family transposase	-
CO57_RS00960	181013..182584	-	1572	WP_065225491.1	IS66 family transposase	-
CO57_RS00965	182604..182951	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CO57_RS00970	182951..183601	-	651	WP_040091510.1	IS66 family insertion sequence hypothetical protein	-
CO57_RS00975	183922..184080	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	184124..184348	-	225	NuclAT_0	-	Antitoxin
-	184124..184348	-	225	NuclAT_0	-	Antitoxin
-	184124..184348	-	225	NuclAT_0	-	Antitoxin
-	184124..184348	-	225	NuclAT_0	-	Antitoxin
CO57_RS31320	184160..184348	+	189	WP_001355331.1	hypothetical protein	-
CO57_RS00985	184360..185079	-	720	WP_000116353.1	plasmid SOS inhibition protein A	-
CO57_RS00990	185076..185510	-	435	WP_000845944.1	conjugation system SOS inhibitor PsiB	-
CO57_RS00995	185579..187543	-	1965	WP_000117198.1	ParB/RepB/Spo0J family partition protein	-
CO57_RS01000	187607..187840	-	234	WP_000005990.1	DUF905 family protein	-
CO57_RS01005	187898..188350	-	453	WP_000290856.1	single-stranded DNA-binding protein	-
CO57_RS31485	188992..189211	-	220	Protein_202	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeJ / faeI / faeH / faeF / faeE / faeD / faeC / hlyD / hlyB / hlyA / hlyC	1..223952	223952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T56506 WP_001312861.1 NZ_CP012499:c184080-183922 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T56506 NZ_CP012499:c184080-183922 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT56506 NZ_CP012499:c184348-184124 [Escherichia coli]
TCACACGGATTGCCCGTGAACTGGCTGAACGACAAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGTAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin