Detailed information of TA system TA056501

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 137908..138161
• Replicon: plasmid pCFSAN004181P
• Accession: NZ_CP012499
• Organism: Escherichia coli strain GB089

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CO57_RS00710
• Protein ID: WP_001312851.1
• Coordinates: 137908..138057 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	138105..138161 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO57_RS00680	133424..133888	-	465	WP_040090730.1	hypothetical protein	-
CO57_RS00685	134167..135207	-	1041	WP_136759863.1	hypothetical protein	-
CO57_RS00695	136208..137065	-	858	WP_000829162.1	incFII family plasmid replication initiator RepA	-
CO57_RS00700	137058..137132	-	75	WP_001364037.1	RepA leader peptide Tap	-
CO57_RS00705	137376..137624	-	249	WP_000083838.1	replication regulatory protein RepA	-
CO57_RS00710	137908..138057	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	138105..138161	+	57	NuclAT_1	-	Antitoxin
-	138105..138161	+	57	NuclAT_1	-	Antitoxin
-	138105..138161	+	57	NuclAT_1	-	Antitoxin
-	138105..138161	+	57	NuclAT_1	-	Antitoxin
CO57_RS00715	138313..138777	-	465	WP_000455478.1	hypothetical protein	-
CO57_RS00720	138932..139522	-	591	WP_000766788.1	DUF2726 domain-containing protein	-
CO57_RS00725	139560..139769	-	210	WP_001355319.1	hemolysin expression modulator Hha	-
CO57_RS00730	140097..140309	-	213	WP_001364091.1	hypothetical protein	-
CO57_RS00735	140444..141004	-	561	WP_000139355.1	fertility inhibition protein FinO	-
CO57_RS00740	141059..141796	-	738	WP_000429592.1	conjugal transfer pilus acetylase TraX	-
CO57_RS00745	141818..142249	-	432	WP_000447372.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeJ / faeI / faeH / faeF / faeE / faeD / faeC / hlyD / hlyB / hlyA / hlyC	1..223952	223952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T56501 WP_001312851.1 NZ_CP012499:c138057-137908 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T56501 NZ_CP012499:c138057-137908 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCTGTGTGCGCTACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT56501 NZ_CP012499:138105-138161 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATCTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin