Detailed information of TA system TA056500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 118764..119335
• Replicon: plasmid pCFSAN004181P
• Accession: NZ_CP012499
• Organism: Escherichia coli strain GB089

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B0VD50
• Locus tag: CO57_RS00630
• Protein ID: WP_024166187.1
• Coordinates: 119054..119335 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: CO57_RS00625
• Protein ID: WP_001081196.1
• Coordinates: 118764..119045 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CO57_RS29805	114122..114816	+	695	WP_085948397.1	IS1 family transposase	-
CO57_RS30600	115152..115428	+	277	Protein_118	transposase	-
CO57_RS00610	115696..116700	-	1005	WP_001179712.1	IS110 family transposase	-
CO57_RS00615	116749..117707	+	959	Protein_120	IS3 family transposase	-
CO57_RS00620	117860..118514	+	655	Protein_121	helix-turn-helix domain-containing protein	-
CO57_RS00625	118764..119045	-	282	WP_001081196.1	HigA family addiction module antidote protein	Antitoxin
CO57_RS00630	119054..119335	-	282	WP_024166187.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CO57_RS00635	119475..120029	-	555	Protein_124	IS21-like element ISEc10 family helper ATPase IstB	-
CO57_RS00640	120025..120807	-	783	Protein_125	hypothetical protein	-
CO57_RS31310	120856..121011	-	156	WP_000074142.1	hypothetical protein	-
CO57_RS00645	121619..122650	+	1032	WP_065224993.1	IS630 family transposase	-
CO57_RS28015	122699..122905	+	207	WP_071961352.1	hypothetical protein	-
CO57_RS30605	122913..123314	+	402	WP_052319587.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	faeJ / faeI / faeH / faeF / faeE / faeD / faeC / hlyD / hlyB / hlyA / hlyC	1..223952	223952
	-	inside	IScluster/Tn	-	-	99453..122650	23197

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10616.24 Da
• Isoelectric Point: 10.4126

>T56500 WP_024166187.1 NZ_CP012499:c119335-119054 [Escherichia coli]
MIKSFRHKGLEHFFKTGSTAGIQAKHAVKLRIQLTALNMAKRPDDMSAPGWKLHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T56500 NZ_CP012499:c119335-119054 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACACTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTAACCGCCCTTAATATGGCTAAACGCCCAGACGATATGTCTGCACCAGGCTGGAAATTAC
ACCCTCTGAAGGGAGCTGATCTTAAAGGCCATTGGGCTATATCTGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGTGAAGACGCAATTCTGGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10191.65 Da
• Isoelectric Point: 7.4026

>AT56500 WP_001081196.1 NZ_CP012499:c119045-118764 [Escherichia coli]
MNRMHNPAHPGAVLREYLGEMSVTDAATALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TARPNIRPLFHHA

Nucleotide

• Length: 282 bp

>AT56500 NZ_CP012499:c119045-118764 [Escherichia coli]
ATGAATCGTATGCATAATCCCGCGCACCCAGGCGCGGTTCTTCGTGAATATCTGGGAGAAATGTCAGTCACAGACGCTGC
CACCGCATTAGGCATTACTCGCACGGCGCTTTCCCGGATTCTGAATGAAAAAGCTGGTATCTCTGCGGATATGGCGCTCC
GCCTGGAATCCGCACTCGGAACTAGCGCCGAAATGTGGACTGGTATGCAATCTCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCGCTCGCCCAAACATTCGCCCATTATTTCACCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0B0VD50