Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 58133..58668 Replicon plasmid ISMMS_VRE7_p1
Accession NZ_CP012461
Organism Enterococcus faecium strain ISMMS_VRE_7

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag AL020_RS14230 Protein ID WP_002305108.1
Coordinates 58396..58668 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag AL020_RS14225 Protein ID WP_002303154.1
Coordinates 58133..58396 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL020_RS14195 54136..54408 + 273 WP_002305107.1 DUF3884 family protein -
AL020_RS14200 54429..55328 + 900 WP_002303235.1 aldose 1-epimerase family protein -
AL020_RS14215 57042..57605 + 564 Protein_61 IS6 family transposase -
AL020_RS14220 57611..58027 + 417 WP_002303153.1 hypothetical protein -
AL020_RS14225 58133..58396 + 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AL020_RS14230 58396..58668 + 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
AL020_RS14235 58745..59074 + 330 WP_002292678.1 AbrB family transcriptional regulator -
AL020_RS14240 59064..59351 + 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
AL020_RS14245 59637..60494 + 858 WP_002292680.1 AAA family ATPase -
AL020_RS14250 60495..61043 + 549 WP_002292681.1 hypothetical protein -
AL020_RS14255 61340..62287 - 948 WP_002303155.1 DNA-binding transcriptional regulator -
AL020_RS14260 62475..63662 + 1188 WP_002303156.1 NADP-dependent malic enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..200151 200151


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T56481 WP_002305108.1 NZ_CP012461:58396-58668 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T56481 NZ_CP012461:58396-58668 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT56481 WP_002303154.1 NZ_CP012461:58133-58396 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT56481 NZ_CP012461:58133-58396 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References