56475

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 179222..179757 Replicon chromosome
Accession NZ_CP012430
Organism Enterococcus faecium strain ISMMS_VRE_1

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag AL014_RS00905 Protein ID WP_002314366.1
Coordinates 179485..179757 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag AL014_RS00900 Protein ID WP_002297422.1
Coordinates 179222..179485 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AL014_RS00885 174650..176899 + 2250 WP_002314387.1 alpha-galactosidase -
AL014_RS00890 176902..178077 + 1176 WP_002336205.1 MFS transporter -
AL014_RS00895 178080..178988 - 909 Protein_181 IS982 family transposase -
AL014_RS00900 179222..179485 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AL014_RS00905 179485..179757 + 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
AL014_RS00910 179834..180163 + 330 WP_002292678.1 AbrB family transcriptional regulator -
AL014_RS00915 180153..180439 + 287 Protein_185 type II toxin-antitoxin system RelE/ParE family toxin -
AL014_RS00920 180725..181582 + 858 WP_002292680.1 AAA family ATPase -
AL014_RS00925 181583..181771 + 189 Protein_187 hypothetical protein -
AL014_RS00930 181830..182510 - 681 WP_002331383.1 IS6-like element IS1216 family transposase -
AL014_RS16310 182586..183229 - 644 Protein_189 transposase -
AL014_RS00945 183504..183740 + 237 Protein_190 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element aac(6')-aph(2'') - 459..291823 291364
- inside IScluster/Tn - - 168621..192708 24087


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T56475 WP_002314366.1 NZ_CP012430:179485-179757 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T56475 NZ_CP012430:179485-179757 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT56475 WP_002297422.1 NZ_CP012430:179222-179485 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT56475 NZ_CP012430:179222-179485 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References