Detailed information of TA system TA056418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1385933..1386530
• Replicon: chromosome
• Accession: NZ_CP012394
• Organism: Neisseria meningitidis strain WUE2121

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: WUE2121_RS07240
• Protein ID: WP_002242826.1
• Coordinates: 1386252..1386530 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WUE2121_RS07235
• Protein ID: WP_002221020.1
• Coordinates: 1385933..1386244 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WUE2121_RS07215	1381171..1381497	+	327	WP_002238081.1	GPW/gp25 family protein	-
WUE2121_RS07220	1381518..1382405	+	888	WP_002238082.1	baseplate J/gp47 family protein	-
WUE2121_RS13810	1382405..1382983	+	579	WP_002238083.1	phage tail protein I	-
WUE2121_RS07230	1383024..1385282	+	2259	WP_154021105.1	pyocin knob domain-containing protein	-
WUE2121_RS12580	1385279..1385407	-	129	Protein_1375	PT domain-containing protein	-
WUE2121_RS07235	1385933..1386244	-	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
WUE2121_RS07240	1386252..1386530	-	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WUE2121_RS07245	1386615..1387220	+	606	WP_002219301.1	hypothetical protein	-
WUE2121_RS07250	1387199..1387495	+	297	WP_002242836.1	hypothetical protein	-
WUE2121_RS14320	1387492..1387659	+	168	WP_014575334.1	hypothetical protein	-
WUE2121_RS07255	1387722..1388909	+	1188	WP_065826137.1	phage tail sheath subtilisin-like domain-containing protein	-
WUE2121_RS07260	1388952..1389458	+	507	WP_014575332.1	phage major tail tube protein	-
WUE2121_RS07265	1389505..1389864	+	360	WP_002238090.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1361467..1429941	68474

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T56418 WP_002242826.1 NZ_CP012394:c1386530-1386252 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T56418 NZ_CP012394:c1386530-1386252 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT56418 WP_002221020.1 NZ_CP012394:c1386244-1385933 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT56418 NZ_CP012394:c1386244-1385933 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6