Detailed information of TA system TA056417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1037687..1038284
• Replicon: chromosome
• Accession: NZ_CP012394
• Organism: Neisseria meningitidis strain WUE2121

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: WUE2121_RS05545
• Protein ID: WP_002242826.1
• Coordinates: 1038006..1038284 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WUE2121_RS05540
• Protein ID: WP_002221020.1
• Coordinates: 1037687..1037998 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WUE2121_RS05520	1032711..1033676	-	966	WP_002221064.1	IS30-like element IS1655 family transposase	-
WUE2121_RS13720	1033741..1033905	+	165	WP_002224559.1	baseplate J/gp47 family protein	-
WUE2121_RS05525	1033905..1034516	+	612	WP_002224558.1	baseplate J/gp47 family protein	-
WUE2121_RS05530	1034513..1035073	+	561	WP_002213590.1	YmfQ family protein	-
WUE2121_RS05535	1035084..1037060	+	1977	WP_065826135.1	pyocin knob domain-containing protein	-
WUE2121_RS12285	1037057..1037173	-	117	Protein_1047	PT domain-containing protein	-
WUE2121_RS05540	1037687..1037998	-	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
WUE2121_RS05545	1038006..1038284	-	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WUE2121_RS05550	1038369..1038974	+	606	WP_002219301.1	hypothetical protein	-
WUE2121_RS05555	1038953..1039249	+	297	WP_002221017.1	hypothetical protein	-
WUE2121_RS05560	1039246..1039455	+	210	WP_002222487.1	hypothetical protein	-
WUE2121_RS05565	1039565..1040708	-	1144	Protein_1053	ABC transporter ATP-binding protein/permease	-
WUE2121_RS05580	1041150..1041797	-	648	WP_002217218.1	DUF799 domain-containing protein	-
WUE2121_RS05585	1041794..1042162	-	369	WP_002219297.1	DUF4810 domain-containing protein	-
WUE2121_RS05590	1042172..1042843	-	672	WP_002217215.1	CsgG/HfaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1023514..1039249	15735
	-	flank	IS/Tn	-	-	1032711..1033676	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T56417 WP_002242826.1 NZ_CP012394:c1038284-1038006 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T56417 NZ_CP012394:c1038284-1038006 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCCGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT56417 WP_002221020.1 NZ_CP012394:c1037998-1037687 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT56417 NZ_CP012394:c1037998-1037687 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6