Detailed information of TA system TA056415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1040181..1040778
• Replicon: chromosome
• Accession: NZ_CP012393
• Organism: Neisseria meningitidis strain DE8555

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0Y6JZK6
• Locus tag: DE8555_RS05550
• Protein ID: WP_002242826.1
• Coordinates: 1040500..1040778 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DE8555_RS05545
• Protein ID: WP_002221020.1
• Coordinates: 1040181..1040492 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DE8555_RS13755	1036062..1036226	+	165	WP_002224559.1	baseplate J/gp47 family protein	-
DE8555_RS05530	1036226..1036837	+	612	WP_002224558.1	baseplate J/gp47 family protein	-
DE8555_RS05535	1036834..1037394	+	561	WP_002213590.1	YmfQ family protein	-
DE8555_RS05540	1037405..1039378	+	1974	WP_063399752.1	pyocin knob domain-containing protein	-
DE8555_RS05545	1040181..1040492	-	312	WP_002221020.1	HigA family addiction module antidote protein	Antitoxin
DE8555_RS05550	1040500..1040778	-	279	WP_002242826.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DE8555_RS05555	1040863..1041468	+	606	WP_061370312.1	hypothetical protein	-
DE8555_RS05560	1041447..1041743	+	297	WP_002221017.1	hypothetical protein	-
DE8555_RS05565	1041740..1041949	+	210	WP_002222487.1	hypothetical protein	-
DE8555_RS05570	1042059..1043202	-	1144	Protein_1052	ABC transporter ATP-binding protein/permease	-
DE8555_RS05585	1043644..1044291	-	648	WP_002217218.1	DUF799 domain-containing protein	-
DE8555_RS05590	1044288..1044656	-	369	WP_002219297.1	DUF4810 domain-containing protein	-
DE8555_RS05595	1044666..1045337	-	672	WP_002217215.1	CsgG/HfaB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1025835..1041743	15908
	-	flank	IS/Tn	-	-	1035032..1035997	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (3-80)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10237.66 Da
• Isoelectric Point: 10.0980

>T56415 WP_002242826.1 NZ_CP012393:c1040778-1040500 [Neisseria meningitidis]
MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPSDMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFND
GNAEVVDYQDYH

Nucleotide

• Length: 279 bp

>T56415 NZ_CP012393:c1040778-1040500 [Neisseria meningitidis]
ATGATTGTTTCTTTCAAACACAAAGGGCTTGAACGCTTTTTTAAAACAGGCTCGCTATCGGGCATCCAAGCTGGGCATTC
CGTCAAACTGAATCTGCTTCTAACCGCGCTGAATGCCGCGCAAACGCCTTCGGATATGGCTGTGCCGAGTTGGAATCTTC
ATCCCCTCAAGGGCAGTTTGTCGGGACATTGGGCGGTTAAAGTCAACGGAAATTGGCGTTTGACTTTTCGTTTCAACGAC
GGCAATGCCGAAGTTGTCGATTATCAGGATTATCACTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11476.32 Da
• Isoelectric Point: 10.5523

>AT56415 WP_002221020.1 NZ_CP012393:c1040492-1040181 [Neisseria meningitidis]
MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQSSITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQR
KTFDIAPLFPEPPLSSMQTGARA

Nucleotide

• Length: 312 bp

>AT56415 NZ_CP012393:c1040492-1040181 [Neisseria meningitidis]
ATGAAAATGCACAATCCTGCCCACCCGGGCCTTGTTTTAAAAGAGTATATTAACGGTGCAAGCATTACCGATATAGCGAA
ACGCCTGGGTGTCAGCCGGGTTGCCTTATCGCGCATTGTCAACGCCCAATCTTCGATTACGCCCGAGATGGCGGTTCGTT
TAAGCCAACTGCTCAATACTTCGCCTGATTTTTGGCTGAATATGCAGGCCGGTTATGATTTGTGGCAAATCAGGCAACGC
AAAACTTTTGATATTGCGCCCTTATTTCCCGAACCGCCTTTGTCTTCGATGCAAACAGGTGCAAGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0Y6JZK6