Detailed information of TA system TA056409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4310575..4311177
• Replicon: chromosome
• Accession: NZ_CP012380
• Organism: Escherichia coli strain WAT

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: AKO64_RS22445
• Protein ID: WP_000897305.1
• Coordinates: 4310575..4310886 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AKO64_RS22450
• Protein ID: WP_000356397.1
• Coordinates: 4310887..4311177 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKO64_RS22415	4305605..4306390	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
AKO64_RS22420	4306489..4307088	+	600	WP_001295269.1	glucose-1-phosphatase	-
AKO64_RS22425	4307082..4307954	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AKO64_RS22430	4307951..4308388	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AKO64_RS22435	4308433..4309374	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AKO64_RS22440	4309438..4310346	-	909	WP_001162704.1	alpha/beta hydrolase	-
AKO64_RS22445	4310575..4310886	+	312	WP_000897305.1	hypothetical protein	Toxin
AKO64_RS22450	4310887..4311177	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AKO64_RS22460	4311782..4312000	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
AKO64_RS22465	4312220..4312462	+	243	WP_001087409.1	hypothetical protein	-
AKO64_RS22470	4312792..4313721	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
AKO64_RS22475	4313718..4314353	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AKO64_RS22480	4314350..4315252	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T56409 WP_000897305.1 NZ_CP012380:4310575-4310886 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T56409 NZ_CP012380:4310575-4310886 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT56409 WP_000356397.1 NZ_CP012380:4310887-4311177 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT56409 NZ_CP012380:4310887-4311177 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7