Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2182217..2182719 Replicon chromosome
Accession NZ_CP012380
Organism Escherichia coli strain WAT

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AKO64_RS11450 Protein ID WP_000767829.1
Coordinates 2182217..2182471 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AKO64_RS11455 Protein ID WP_001259255.1
Coordinates 2182468..2182719 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKO64_RS11425 2177232..2177459 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AKO64_RS11430 2177473..2178531 - 1059 WP_000492324.1 transport protein YeeE -
AKO64_RS11435 2178710..2180068 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AKO64_RS25340 2180058..2180120 - 63 WP_010723108.1 membrane protein YoeI -
AKO64_RS11440 2180335..2181264 - 930 WP_023154044.1 LysR family transcriptional regulator -
AKO64_RS11445 2181310..2182134 - 825 WP_000754758.1 SDR family oxidoreductase -
AKO64_RS11450 2182217..2182471 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AKO64_RS11455 2182468..2182719 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AKO64_RS11460 2183002..2183052 + 51 WP_001364200.1 his operon leader peptide -
AKO64_RS11470 2183198..2184097 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AKO64_RS11475 2184103..2185407 + 1305 WP_021523144.1 histidinol dehydrogenase -
AKO64_RS11480 2185404..2186474 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
AKO64_RS11485 2186474..2187541 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T56403 WP_000767829.1 NZ_CP012380:c2182471-2182217 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T56403 NZ_CP012380:c2182471-2182217 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT56403 WP_001259255.1 NZ_CP012380:c2182719-2182468 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT56403 NZ_CP012380:c2182719-2182468 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References