56403

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2182217..2182719 Replicon chromosome
Accession NZ_CP012380
Organism Escherichia coli strain WAT

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AKO64_RS11450 Protein ID WP_000767829.1
Coordinates 2182217..2182471 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AKO64_RS11455 Protein ID WP_001259255.1
Coordinates 2182468..2182719 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKO64_RS11425 2177232..2177459 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
AKO64_RS11430 2177473..2178531 - 1059 WP_000492324.1 transport protein YeeE -
AKO64_RS11435 2178710..2180068 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AKO64_RS25340 2180058..2180120 - 63 WP_010723108.1 membrane protein YoeI -
AKO64_RS11440 2180335..2181264 - 930 WP_023154044.1 LysR family transcriptional regulator -
AKO64_RS11445 2181310..2182134 - 825 WP_000754758.1 SDR family oxidoreductase -
AKO64_RS11450 2182217..2182471 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AKO64_RS11455 2182468..2182719 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AKO64_RS11460 2183002..2183052 + 51 WP_001364200.1 his operon leader peptide -
AKO64_RS11470 2183198..2184097 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
AKO64_RS11475 2184103..2185407 + 1305 WP_021523144.1 histidinol dehydrogenase -
AKO64_RS11480 2185404..2186474 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
AKO64_RS11485 2186474..2187541 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T56403 WP_000767829.1 NZ_CP012380:c2182471-2182217 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T56403 NZ_CP012380:c2182471-2182217 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT56403 WP_001259255.1 NZ_CP012380:c2182719-2182468 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT56403 NZ_CP012380:c2182719-2182468 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References