Detailed information of TA system TA056381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4253098..4253700
• Replicon: chromosome
• Accession: NZ_CP012379
• Organism: Escherichia coli strain PAR

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: AKO63_RS21670
• Protein ID: WP_000897302.1
• Coordinates: 4253098..4253409 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AKO63_RS21675
• Protein ID: WP_000356397.1
• Coordinates: 4253410..4253700 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKO63_RS21645	4249012..4249611	+	600	WP_001314324.1	glucose-1-phosphatase	-
AKO63_RS21650	4249605..4250477	+	873	WP_000920762.1	virulence factor BrkB family protein	-
AKO63_RS21655	4250474..4250911	+	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
AKO63_RS21660	4250956..4251897	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
AKO63_RS21665	4251961..4252869	-	909	WP_001331553.1	alpha/beta hydrolase	-
AKO63_RS21670	4253098..4253409	+	312	WP_000897302.1	hypothetical protein	Toxin
AKO63_RS21675	4253410..4253700	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
AKO63_RS21685	4254059..4254337	+	279	WP_001296612.1	hypothetical protein	-
AKO63_RS21695	4254733..4254951	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
AKO63_RS21700	4255167..4256096	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
AKO63_RS21705	4256093..4256728	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
AKO63_RS21710	4256725..4257627	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T56381 WP_000897302.1 NZ_CP012379:4253098-4253409 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T56381 NZ_CP012379:4253098-4253409 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT56381 WP_000356397.1 NZ_CP012379:4253410-4253700 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT56381 NZ_CP012379:4253410-4253700 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416