Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3426241..3427040 Replicon chromosome
Accession NZ_CP012379
Organism Escherichia coli strain PAR

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag AKO63_RS17490 Protein ID WP_000347251.1
Coordinates 3426576..3427040 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag AKO63_RS17485 Protein ID WP_001296435.1
Coordinates 3426241..3426576 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKO63_RS17470 3422026..3422796 - 771 WP_001058223.1 2-dehydro-3-deoxyglucarate aldolase -
AKO63_RS17475 3422812..3424146 - 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
AKO63_RS17480 3424521..3426092 + 1572 WP_001539800.1 galactarate dehydratase -
AKO63_RS17485 3426241..3426576 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
AKO63_RS17490 3426576..3427040 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
AKO63_RS17495 3427095..3427904 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
AKO63_RS17505 3428153..3429433 + 1281 WP_000681931.1 tagatose-bisphosphate aldolase subunit KbaZ -
AKO63_RS17510 3429456..3429929 + 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
AKO63_RS17515 3429940..3430719 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
AKO63_RS17520 3430709..3431587 + 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
AKO63_RS17525 3431605..3432039 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T56380 WP_000347251.1 NZ_CP012379:3426576-3427040 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T56380 NZ_CP012379:3426576-3427040 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT56380 WP_001296435.1 NZ_CP012379:3426241-3426576 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT56380 NZ_CP012379:3426241-3426576 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References