Detailed information of TA system TA056376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2848258..2848985
• Replicon: chromosome
• Accession: NZ_CP012379
• Organism: Escherichia coli strain PAR

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: AKO63_RS14660
• Protein ID: WP_000547563.1
• Coordinates: 2848674..2848985 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AKO63_RS14655
• Protein ID: WP_000126304.1
• Coordinates: 2848258..2848677 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKO63_RS14635	2844421..2846673	-	2253	WP_064238339.1	carbamoyltransferase HypF	-
AKO63_RS14640	2846826..2847353	-	528	WP_001078777.1	electron transport protein HydN	-
AKO63_RS14650	2847738..2848166	+	429	WP_000536064.1	hypothetical protein	-
AKO63_RS14655	2848258..2848677	-	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
AKO63_RS14660	2848674..2848985	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
AKO63_RS14665	2849148..2849903	-	756	WP_001305268.1	hypothetical protein	-
AKO63_RS14670	2849946..2850416	-	471	WP_000132956.1	hydrogenase maturation peptidase HycI	-
AKO63_RS14675	2850409..2850819	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
AKO63_RS14680	2850816..2851583	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
AKO63_RS14685	2851583..2852125	-	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
AKO63_RS14690	2852135..2853844	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T56376 WP_000547563.1 NZ_CP012379:c2848985-2848674 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T56376 NZ_CP012379:c2848985-2848674 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGCAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT56376 WP_000126304.1 NZ_CP012379:c2848677-2848258 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT56376 NZ_CP012379:c2848677-2848258 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22