Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2124230..2124732 Replicon chromosome
Accession NZ_CP012379
Organism Escherichia coli strain PAR

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag AKO63_RS11125 Protein ID WP_000767822.1
Coordinates 2124230..2124484 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AKO63_RS11130 Protein ID WP_001259255.1
Coordinates 2124481..2124732 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AKO63_RS11110 2120723..2122081 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
AKO63_RS24890 2122071..2122133 - 63 WP_010723108.1 membrane protein YoeI -
AKO63_RS11115 2122348..2123277 - 930 WP_000803366.1 LysR family transcriptional regulator -
AKO63_RS11120 2123323..2124147 - 825 WP_000754737.1 SDR family oxidoreductase -
AKO63_RS11125 2124230..2124484 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AKO63_RS11130 2124481..2124732 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AKO63_RS11135 2125015..2125065 + 51 WP_001364200.1 his operon leader peptide -
AKO63_RS11145 2125211..2126110 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
AKO63_RS11150 2126116..2127420 + 1305 WP_001445483.1 histidinol dehydrogenase -
AKO63_RS11155 2127417..2128487 + 1071 WP_001538918.1 histidinol-phosphate transaminase -
AKO63_RS11160 2128487..2129554 + 1068 WP_001538920.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T56375 WP_000767822.1 NZ_CP012379:c2124484-2124230 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T56375 NZ_CP012379:c2124484-2124230 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT56375 WP_001259255.1 NZ_CP012379:c2124732-2124481 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT56375 NZ_CP012379:c2124732-2124481 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References