Detailed information of TA system TA056350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3503804..3504603
• Replicon: chromosome
• Accession: NZ_CP012376
• Organism: Escherichia coli strain GE3

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F4VJD3
• Locus tag: AKN41_RS17140
• Protein ID: WP_000347266.1
• Coordinates: 3504139..3504603 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: AKN41_RS17135
• Protein ID: WP_001307405.1
• Coordinates: 3503804..3504139 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AKN41_RS17120	3499589..3500359	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
AKN41_RS17125	3500375..3501709	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
AKN41_RS17130	3502084..3503655	+	1572	WP_001273741.1	galactarate dehydratase	-
AKN41_RS17135	3503804..3504139	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
AKN41_RS17140	3504139..3504603	+	465	WP_000347266.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
AKN41_RS17145	3504658..3505467	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
AKN41_RS17150	3505716..3506996	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
AKN41_RS17155	3507019..3507492	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
AKN41_RS17160	3507503..3508282	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
AKN41_RS17165	3508272..3509150	+	879	WP_001315856.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
AKN41_RS17170	3509168..3509602	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3492816..3504603	11787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17841.18 Da
• Isoelectric Point: 9.4947

>T56350 WP_000347266.1 NZ_CP012376:3504139-3504603 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T56350 NZ_CP012376:3504139-3504603 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT56350 WP_001307405.1 NZ_CP012376:3503804-3504139 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT56350 NZ_CP012376:3503804-3504139 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A836NGD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7